Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 332594..333180 Replicon chromosome
Accession NZ_CP040354
Organism Klebsiella pneumoniae strain P20

Toxin (Protein)


Gene name doc Uniprot ID A0A7Z9B6M3
Locus tag FEZ11_RS01540 Protein ID WP_029884514.1
Coordinates 332812..333180 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag FEZ11_RS01535 Protein ID WP_004174006.1
Coordinates 332594..332815 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FEZ11_RS01515 (328751) 328751..329677 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
FEZ11_RS01520 (329674) 329674..330951 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
FEZ11_RS01525 (330948) 330948..331715 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
FEZ11_RS01530 (331717) 331717..332430 + 714 WP_029884513.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
FEZ11_RS01535 (332594) 332594..332815 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
FEZ11_RS01540 (332812) 332812..333180 + 369 WP_029884514.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
FEZ11_RS01545 (333453) 333453..334769 + 1317 WP_029884515.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
FEZ11_RS01550 (334876) 334876..335763 + 888 WP_004901104.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
FEZ11_RS01555 (335760) 335760..336605 + 846 WP_004901101.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
FEZ11_RS01560 (336607) 336607..337677 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 329674..338414 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(6-116)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13583.01 Da        Isoelectric Point: 8.6410

>T125617 WP_029884514.1 NZ_CP040354:332812-333180 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRMALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T125617 NZ_CP040354:332812-333180 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCATGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTATGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT125617 WP_004174006.1 NZ_CP040354:332594-332815 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT125617 NZ_CP040354:332594-332815 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Z9B6M3

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References