Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 353150..353796 Replicon chromosome
Accession NZ_CP040351
Organism Klebsiella pneumoniae strain P39

Toxin (Protein)


Gene name higB Uniprot ID W9BBY1
Locus tag FEZ16_RS01635 Protein ID WP_016529833.1
Coordinates 353150..353497 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag FEZ16_RS01640 Protein ID WP_002920557.1
Coordinates 353497..353796 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FEZ16_RS01625 (FEZ16_01625) 349076..350509 + 1434 WP_130974867.1 glycogen synthase GlgA -
FEZ16_RS01630 (FEZ16_01630) 350527..352974 + 2448 WP_021312822.1 glycogen phosphorylase -
FEZ16_RS01635 (FEZ16_01635) 353150..353497 + 348 WP_016529833.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FEZ16_RS01640 (FEZ16_01640) 353497..353796 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
FEZ16_RS01645 (FEZ16_01645) 353859..355367 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
FEZ16_RS01650 (FEZ16_01650) 355572..355901 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
FEZ16_RS01655 (FEZ16_01655) 355952..356782 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
FEZ16_RS01660 (FEZ16_01660) 356832..357590 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13549.56 Da        Isoelectric Point: 5.6749

>T125597 WP_016529833.1 NZ_CP040351:353150-353497 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T125597 NZ_CP040351:353150-353497 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT125597 WP_002920557.1 NZ_CP040351:353497-353796 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT125597 NZ_CP040351:353497-353796 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCAGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W9BBY1

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References