Detailed information of TA system TA125572

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1600907..1601591
• Replicon: chromosome
• Accession: NZ_CP040348
• Organism: Collinsella aerofaciens strain P10wA7

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: A0A096LL49
• Locus tag: FEO44_RS07105
• Protein ID: WP_022094268.1
• Coordinates: 1601196..1601591 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FEO44_RS07100
• Protein ID: WP_006235701.1
• Coordinates: 1600907..1601209 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEO44_RS07070	1595969..1596163	-	195	WP_022094262.1	ATP synthase subunit C	-
FEO44_RS07075	1596288..1597091	-	804	WP_022094263.1	F0F1 ATP synthase subunit A	-
FEO44_RS07080	1597095..1597406	-	312	WP_035138788.1	hypothetical protein	-
FEO44_RS07085	1597689..1598336	-	648	WP_040359658.1	uracil phosphoribosyltransferase	-
FEO44_RS07090	1598460..1600001	-	1542	WP_138113477.1	glycerol kinase GlpK	-
FEO44_RS07095	1600015..1600758	-	744	WP_138113478.1	L-threonylcarbamoyladenylate synthase	-
FEO44_RS07100	1600907..1601209	-	303	WP_006235701.1	XRE family transcriptional regulator	Antitoxin
FEO44_RS07105	1601196..1601591	-	396	WP_022094268.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FEO44_RS07110	1601667..1602941	-	1275	WP_022094269.1	peptide chain release factor N(5)-glutamine methyltransferase	-
FEO44_RS07115	1603574..1604641	-	1068	WP_138113479.1	peptide chain release factor 1	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (18-118)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-82)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 15133.44 Da
• Isoelectric Point: 10.2899

>T125572 WP_022094268.1 NZ_CP040348:c1601591-1601196 [Collinsella aerofaciens]
MSNQLHRYRIVLDYIEPWLDEQDEATLKQIYADLLVLEKEGPSLGRPLVDRVKGSKLHHLKELRVTSCGGQVIRILFAFD
PKRQAVLLLAGDKSRAGSSRAKWNGWYAINIPRAEQIYRRHVRRLDRDGTA

Nucleotide

• Length: 396 bp

>T125572 NZ_CP040348:c1601591-1601196 [Collinsella aerofaciens]
ATGTCAAATCAGCTGCATCGGTACCGTATCGTGCTTGATTACATTGAACCTTGGCTTGATGAGCAGGATGAAGCTACGCT
GAAGCAGATTTATGCAGACCTTTTGGTGCTCGAGAAGGAAGGTCCCAGCCTTGGTCGTCCGCTGGTTGACCGCGTAAAGG
GCTCGAAGCTTCATCATTTAAAGGAGCTGAGAGTGACGTCGTGTGGTGGGCAGGTGATTCGCATCCTCTTCGCCTTTGAC
CCCAAGCGCCAGGCGGTATTGCTATTGGCGGGTGATAAGTCGCGAGCGGGATCGTCAAGGGCAAAATGGAACGGTTGGTA
TGCGATCAACATTCCAAGGGCCGAGCAAATATACCGTCGCCACGTAAGGAGGCTCGATCGAGATGGAACTGCATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11388.92 Da
• Isoelectric Point: 8.5154

>AT125572 WP_006235701.1 NZ_CP040348:c1601209-1600907 [Collinsella aerofaciens]
MELHEYMESRGITRDSITAEQERTRDRIEAYKLAQIRRLKDLTQASVAQSMGVSQKRVSELERGELGSMRLDTLSRYAES
LGGKLVASIEFPDRTVTLAR

Nucleotide

• Length: 303 bp

>AT125572 NZ_CP040348:c1601209-1600907 [Collinsella aerofaciens]
ATGGAACTGCATGAGTATATGGAATCTCGCGGGATAACACGCGACTCCATTACCGCCGAGCAGGAGAGGACTCGCGATCG
TATCGAAGCCTATAAGCTTGCTCAGATTCGCAGGTTAAAAGATCTAACACAGGCGTCGGTCGCCCAGTCGATGGGCGTTT
CTCAAAAACGTGTATCCGAGCTCGAGCGTGGGGAGTTGGGTTCGATGAGGCTCGACACGCTGAGCAGGTACGCAGAGAGC
CTCGGCGGAAAACTGGTTGCAAGCATCGAGTTTCCCGATCGTACCGTTACGCTTGCGCGCTAA

Structures

Toxin

• AlphaFold DB: A0A096LL49