Detailed information of TA system TA125537

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2954320..2954545
• Replicon: chromosome
• Accession: NZ_CP040316
• Organism: Escherichia coli strain CV261

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: FEL12_RS14530
• Protein ID: WP_000967408.1
• Coordinates: 2954320..2954532 (-)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2954487..2954545 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEL12_RS14500	2949641..2950699	-	1059	WP_000483497.1	site-specific DNA-methyltransferase	-
FEL12_RS14505	2950850..2951047	-	198	WP_000917735.1	hypothetical protein	-
FEL12_RS14510	2951274..2952095	-	822	WP_000762904.1	antitermination protein	-
FEL12_RS14515	2952092..2952466	-	375	WP_000904137.1	RusA family crossover junction endodeoxyribonuclease	-
FEL12_RS14520	2952479..2953528	-	1050	WP_001302870.1	DUF968 domain-containing protein	-
FEL12_RS14525	2953530..2953808	-	279	WP_001304183.1	hypothetical protein	-
FEL12_RS27470	2953874..2954041	-	168	WP_000998188.1	hypothetical protein	-
FEL12_RS14530	2954320..2954532	-	213	WP_000967408.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2954487..2954545	+	59	-	-	Antitoxin
FEL12_RS14535	2954721..2954825	-	105	WP_001278450.1	hypothetical protein	-
FEL12_RS14540	2954941..2955303	-	363	WP_000610379.1	DUF551 domain-containing protein	-
FEL12_RS14545	2955300..2955671	-	372	WP_000137941.1	hypothetical protein	-
FEL12_RS14550	2955707..2955919	-	213	WP_000063625.1	hypothetical protein	-
FEL12_RS14555	2955968..2956324	-	357	WP_001302146.1	hypothetical protein	-
FEL12_RS14560	2956381..2956776	-	396	WP_001118161.1	DUF977 family protein	-
FEL12_RS14565	2956792..2957562	-	771	WP_000450888.1	DUF1627 domain-containing protein	-
FEL12_RS14570	2957592..2958332	-	741	WP_000790456.1	ATP-binding protein	-
FEL12_RS14575	2958339..2959292	-	954	WP_000095667.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI	2918375..2964725	46350

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7958.54 Da
• Isoelectric Point: 9.6297

>T125537 WP_000967408.1 NZ_CP040316:c2954532-2954320 [Escherichia coli]
MLYTRRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 213 bp

>T125537 NZ_CP040316:c2954532-2954320 [Escherichia coli]
ATGCTCTACACACGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGCT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTACGAATCC
GAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT125537 NZ_CP040316:2954487-2954545 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTGTGTAGAGCATAGATATGGCCTCA

Structures

Antitoxin