Detailed information of TA system TA125534

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2588238..2588463
• Replicon: chromosome
• Accession: NZ_CP040316
• Organism: Escherichia coli strain CV261

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: FEL12_RS12755
• Protein ID: WP_000935259.1
• Coordinates: 2588251..2588463 (+)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2588238..2588296 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEL12_RS12705	2583344..2583586	+	243	WP_000747948.1	hypothetical protein	-
FEL12_RS12710	2583570..2583995	+	426	WP_000693878.1	Rha family transcriptional regulator	-
FEL12_RS12715	2584064..2585107	+	1044	WP_001262402.1	hypothetical protein	-
FEL12_RS12720	2585100..2585561	+	462	WP_000139447.1	replication protein	-
FEL12_RS12725	2585595..2586270	+	676	Protein_2470	DUF1627 domain-containing protein	-
FEL12_RS12730	2586303..2586584	+	282	WP_000603384.1	DNA-binding protein	-
FEL12_RS12735	2586581..2586808	+	228	WP_000699809.1	hypothetical protein	-
FEL12_RS12740	2586801..2587112	+	312	WP_001289673.1	hypothetical protein	-
FEL12_RS12745	2587240..2587458	+	219	WP_000683609.1	DUF4014 family protein	-
FEL12_RS12750	2587460..2588017	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2588238..2588296	-	59	-	-	Antitoxin
FEL12_RS12755	2588251..2588463	+	213	WP_000935259.1	type I toxin-antitoxin system Hok family toxin	Toxin
FEL12_RS12760	2588583..2588927	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
FEL12_RS12765	2589049..2589321	+	273	WP_000191872.1	hypothetical protein	-
FEL12_RS12770	2589323..2590372	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
FEL12_RS12775	2590385..2590690	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
FEL12_RS12780	2590753..2591307	+	555	WP_000640035.1	DUF1133 family protein	-
FEL12_RS12785	2591532..2591729	+	198	WP_000917763.1	hypothetical protein	-
FEL12_RS12790	2591865..2592578	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
FEL12_RS12805	2593029..2593460	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	paa / nleG7'	2522279..2625567	103288

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7887.47 Da
• Isoelectric Point: 9.2654

>T125534 WP_000935259.1 NZ_CP040316:2588251-2588463 [Escherichia coli]
MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 213 bp

>T125534 NZ_CP040316:2588251-2588463 [Escherichia coli]
ATGCTGAACACATGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTGCGAATCC
GAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT125534 NZ_CP040316:c2588296-2588238 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Antitoxin