Detailed information of TA system TA125529

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1998330..1998555
• Replicon: chromosome
• Accession: NZ_CP040316
• Organism: Escherichia coli strain CV261

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: FEL12_RS09340
• Protein ID: WP_000813263.1
• Coordinates: 1998400..1998555 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1998330..1998388 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEL12_RS09310	1993605..1994696	+	1092	WP_001205823.1	hypothetical protein	-
FEL12_RS09315	1994703..1995449	+	747	WP_000788751.1	ATP-binding protein	-
FEL12_RS09320	1995471..1996241	+	771	WP_000450992.1	DUF1627 domain-containing protein	-
FEL12_RS09325	1996257..1996670	+	414	WP_001151233.1	DUF977 family protein	-
FEL12_RS09330	1997022..1997795	-	774	WP_000160654.1	alpha/beta hydrolase	-
-	1998330..1998388	-	59	-	-	Antitoxin
FEL12_RS09340	1998400..1998555	+	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
FEL12_RS09345	1998723..1999001	+	279	WP_001341388.1	hypothetical protein	-
FEL12_RS09350	1999003..2000052	+	1050	WP_001265172.1	DUF968 domain-containing protein	-
FEL12_RS09355	2000065..2000436	+	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
FEL12_RS09360	2000426..2000797	+	372	WP_000090264.1	antitermination protein	-
FEL12_RS09365	2000949..2001767	+	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
FEL12_RS09370	2002054..2002250	+	197	Protein_1818	TrmB family transcriptional regulator	-
FEL12_RS09375	2002388..2003101	+	714	WP_000261909.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T125529 WP_000813263.1 NZ_CP040316:1998400-1998555 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T125529 NZ_CP040316:1998400-1998555 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT125529 NZ_CP040316:c1998388-1998330 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin