Detailed information of TA system TA125481

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 1036400..1036939
• Replicon: chromosome
• Accession: NZ_CP040314
• Organism: Escherichia coli strain MA11

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: FEL10_RS04735
• Protein ID: WP_001303998.1
• Coordinates: 1036400..1036660 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: C3TP07
• Locus tag: FEL10_RS04740
• Protein ID: WP_000729705.1
• Coordinates: 1036679..1036939 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEL10_RS04720	1034083..1034661	+	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-
FEL10_RS04725	1034766..1035533	+	768	WP_001301698.1	class II glutamine amidotransferase	-
FEL10_RS04730	1035504..1036244	-	741	WP_001225679.1	murein L,D-transpeptidase	-
FEL10_RS04735	1036400..1036660	-	261	WP_001303998.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
FEL10_RS04740	1036679..1036939	-	261	WP_000729705.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
FEL10_RS04745	1037125..1037898	+	774	WP_000543891.1	C40 family peptidase	-
FEL10_RS04750	1038074..1038571	+	498	Protein_912	REP-associated tyrosine transposase RayT	-
FEL10_RS04755	1038716..1040455	-	1740	WP_001301901.1	flagellar biosynthesis protein FlhA	-
FEL10_RS04760	1040400..1041185	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-85)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10026.71 Da
• Isoelectric Point: 10.3188

>T125481 WP_001303998.1 NZ_CP040314:c1036660-1036400 [Escherichia coli]
MNSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGT
HAALFG

Nucleotide

• Length: 261 bp

>T125481 NZ_CP040314:c1036660-1036400 [Escherichia coli]
TTGAACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAATAAATTGAAATATCTTAT
GACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAGGTTCATGGAAAGGTTATC
GCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGATTTGAGAGAACTGGAACT
CACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9377.74 Da
• Isoelectric Point: 5.0758

>AT125481 WP_000729705.1 NZ_CP040314:c1036939-1036679 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Nucleotide

• Length: 261 bp

>AT125481 NZ_CP040314:c1036939-1036679 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA