Detailed information of TA system TA125401

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1046378..1047072
• Replicon: chromosome
• Accession: NZ_CP040311
• Organism: Escherichia coli strain F3398

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: FEL04_RS04760
• Protein ID: WP_001263495.1
• Coordinates: 1046674..1047072 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: FEL04_RS04755
• Protein ID: WP_000554757.1
• Coordinates: 1046378..1046671 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEL04_RS04735	1042089..1042586	+	498	Protein_908	REP-associated tyrosine transposase RayT	-
FEL04_RS04740	1042731..1044470	-	1740	WP_001303130.1	flagellar biosynthesis protein FlhA	-
FEL04_RS04745	1044415..1045200	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
FEL04_RS04750	1045271..1046326	+	1056	WP_001226186.1	DNA polymerase IV	-
FEL04_RS04755	1046378..1046671	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FEL04_RS04760	1046674..1047072	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FEL04_RS04765	1047082..1047534	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
FEL04_RS04770	1047853..1048056	+	204	Protein_915	RNA ligase RtcB family protein	-
FEL04_RS04775	1048055..1048576	+	522	Protein_916	peptide chain release factor H	-
FEL04_RS04780	1048633..1050090	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
FEL04_RS04785	1050351..1050809	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	1051405..1051485	+	81	NuclAT_11	-	-
-	1051405..1051485	+	81	NuclAT_11	-	-
-	1051405..1051485	+	81	NuclAT_11	-	-
-	1051405..1051485	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T125401 WP_001263495.1 NZ_CP040311:1046674-1047072 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T125401 NZ_CP040311:1046674-1047072 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT125401 WP_000554757.1 NZ_CP040311:1046378-1046671 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT125401 NZ_CP040311:1046378-1046671 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6