Detailed information of TA system TA125370

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2874757..2874982
• Replicon: chromosome
• Accession: NZ_CP040309
• Organism: Escherichia coli strain 21B8

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: FEK99_RS13970
• Protein ID: WP_000967408.1
• Coordinates: 2874757..2874969 (-)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2874924..2874982 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEK99_RS13940	2870060..2871118	-	1059	WP_000483497.1	site-specific DNA-methyltransferase	-
FEK99_RS13945	2871269..2871466	-	198	WP_000917735.1	hypothetical protein	-
FEK99_RS13950	2871693..2872514	-	822	WP_000762904.1	antitermination protein	-
FEK99_RS13955	2872511..2872885	-	375	WP_000904137.1	RusA family crossover junction endodeoxyribonuclease	-
FEK99_RS13960	2872898..2873947	-	1050	Protein_2717	DUF968 domain-containing protein	-
FEK99_RS13965	2873949..2874227	-	279	WP_001304183.1	hypothetical protein	-
FEK99_RS27175	2874293..2874460	-	168	WP_000998188.1	hypothetical protein	-
FEK99_RS13970	2874757..2874969	-	213	WP_000967408.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2874924..2874982	+	59	-	-	Antitoxin
FEK99_RS13975	2875158..2875262	-	105	WP_001278450.1	hypothetical protein	-
FEK99_RS13980	2875378..2875740	-	363	WP_000610379.1	DUF551 domain-containing protein	-
FEK99_RS13985	2875737..2876108	-	372	WP_000137941.1	hypothetical protein	-
FEK99_RS13990	2876144..2876356	-	213	WP_000063625.1	hypothetical protein	-
FEK99_RS13995	2876405..2876761	-	357	WP_001302146.1	hypothetical protein	-
FEK99_RS14000	2876818..2877213	-	396	WP_001118161.1	DUF977 family protein	-
FEK99_RS14005	2877229..2877999	-	771	WP_000450888.1	DUF1627 domain-containing protein	-
FEK99_RS14010	2878029..2878769	-	741	WP_000790456.1	ATP-binding protein	-
FEK99_RS14015	2878776..2879729	-	954	WP_000095667.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7' / espM1 / nleA/espI	2791075..2952476	161401
	-	inside	Prophage	-	nleG7' / espM1 / nleA/espI	2789287..2952476	163189

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7958.54 Da
• Isoelectric Point: 9.6297

>T125370 WP_000967408.1 NZ_CP040309:c2874969-2874757 [Escherichia coli]
MLYTRRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 213 bp

>T125370 NZ_CP040309:c2874969-2874757 [Escherichia coli]
ATGCTCTACACACGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGCT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTACGAATCC
GAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT125370 NZ_CP040309:2874924-2874982 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTGTGTAGAGCATAGATATGGCCTCA

Structures

Antitoxin