Detailed information of TA system TA125368

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2828138..2828363
• Replicon: chromosome
• Accession: NZ_CP040309
• Organism: Escherichia coli strain 21B8

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: FEK99_RS13665
• Protein ID: WP_000935259.1
• Coordinates: 2828138..2828350 (-)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2828305..2828363 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEK99_RS13615	2823141..2823572	-	432	WP_001302123.1	tellurite resistance protein	-
FEK99_RS13630	2824023..2824736	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
FEK99_RS13635	2824872..2825069	-	198	WP_000917763.1	hypothetical protein	-
FEK99_RS13640	2825294..2825848	-	555	WP_000640035.1	DUF1133 family protein	-
FEK99_RS13645	2825911..2826216	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
FEK99_RS13650	2826229..2827278	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
FEK99_RS13655	2827280..2827552	-	273	WP_000191872.1	hypothetical protein	-
FEK99_RS13660	2827674..2828018	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
FEK99_RS13665	2828138..2828350	-	213	WP_000935259.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2828305..2828363	+	59	-	-	Antitoxin
FEK99_RS13670	2828584..2829141	-	558	WP_000104474.1	DUF551 domain-containing protein	-
FEK99_RS13675	2829143..2829361	-	219	WP_000683609.1	DUF4014 family protein	-
FEK99_RS13680	2829489..2829800	-	312	WP_001289673.1	hypothetical protein	-
FEK99_RS13685	2829793..2830020	-	228	WP_000699809.1	hypothetical protein	-
FEK99_RS13690	2830017..2830298	-	282	WP_000603384.1	DNA-binding protein	-
FEK99_RS13695	2830331..2831047	-	717	WP_000450627.1	DUF1627 domain-containing protein	-
FEK99_RS13700	2831081..2831542	-	462	WP_000139447.1	replication protein	-
FEK99_RS13705	2831535..2832578	-	1044	WP_001262402.1	hypothetical protein	-
FEK99_RS13710	2832647..2833072	-	426	WP_000693878.1	Rha family transcriptional regulator	-
FEK99_RS13715	2833056..2833298	-	243	WP_000747948.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7' / espM1 / nleA/espI	2791075..2952476	161401
	-	inside	Prophage	-	nleG7' / espM1 / nleA/espI	2789287..2952476	163189

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7887.47 Da
• Isoelectric Point: 9.2654

>T125368 WP_000935259.1 NZ_CP040309:c2828350-2828138 [Escherichia coli]
MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 213 bp

>T125368 NZ_CP040309:c2828350-2828138 [Escherichia coli]
ATGCTGAACACATGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTGCGAATCC
GAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT125368 NZ_CP040309:2828305-2828363 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Antitoxin