Detailed information of TA system TA125362

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1951975..1952200
• Replicon: chromosome
• Accession: NZ_CP040309
• Organism: Escherichia coli strain 21B8

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: FEK99_RS09050
• Protein ID: WP_000813263.1
• Coordinates: 1952045..1952200 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1951975..1952033 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEK99_RS09020	1947250..1948341	+	1092	WP_001205823.1	hypothetical protein	-
FEK99_RS09025	1948348..1949094	+	747	WP_000788751.1	ATP-binding protein	-
FEK99_RS09030	1949116..1949886	+	771	WP_000450992.1	DUF1627 domain-containing protein	-
FEK99_RS09035	1949902..1950315	+	414	WP_001151233.1	DUF977 family protein	-
FEK99_RS09040	1950667..1951440	-	774	WP_000160654.1	alpha/beta hydrolase	-
-	1951975..1952033	-	59	-	-	Antitoxin
FEK99_RS09050	1952045..1952200	+	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
FEK99_RS09055	1952368..1952646	+	279	WP_001341388.1	hypothetical protein	-
FEK99_RS09060	1952648..1953697	+	1050	WP_001265172.1	DUF968 domain-containing protein	-
FEK99_RS09065	1953710..1954081	+	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
FEK99_RS09070	1954071..1954442	+	372	WP_000090264.1	antitermination protein	-
FEK99_RS09075	1954594..1955412	+	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
FEK99_RS09080	1955699..1955895	+	197	Protein_1762	TrmB family transcriptional regulator	-
FEK99_RS09085	1956033..1956746	+	714	WP_000261909.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T125362 WP_000813263.1 NZ_CP040309:1952045-1952200 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T125362 NZ_CP040309:1952045-1952200 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT125362 NZ_CP040309:c1952033-1951975 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin