Detailed information of TA system TA125358

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1038550..1039244
• Replicon: chromosome
• Accession: NZ_CP040309
• Organism: Escherichia coli strain 21B8

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: FEK99_RS04745
• Protein ID: WP_001263495.1
• Coordinates: 1038846..1039244 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: FEK99_RS04740
• Protein ID: WP_000554757.1
• Coordinates: 1038550..1038843 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEK99_RS04720	1034261..1034758	+	498	Protein_906	REP-associated tyrosine transposase RayT	-
FEK99_RS04725	1034903..1036642	-	1740	WP_001301901.1	flagellar biosynthesis protein FlhA	-
FEK99_RS04730	1036587..1037372	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
FEK99_RS04735	1037443..1038498	+	1056	WP_001226186.1	DNA polymerase IV	-
FEK99_RS04740	1038550..1038843	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FEK99_RS04745	1038846..1039244	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FEK99_RS04750	1039254..1039706	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
FEK99_RS04755	1040025..1040228	+	204	Protein_913	RNA ligase RtcB family protein	-
FEK99_RS04760	1040227..1040748	+	522	Protein_914	peptide chain release factor H	-
FEK99_RS04765	1040805..1042262	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
FEK99_RS04770	1042523..1042981	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	1043577..1043657	+	81	NuclAT_11	-	-
-	1043577..1043657	+	81	NuclAT_11	-	-
-	1043577..1043657	+	81	NuclAT_11	-	-
-	1043577..1043657	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T125358 WP_001263495.1 NZ_CP040309:1038846-1039244 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T125358 NZ_CP040309:1038846-1039244 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT125358 WP_000554757.1 NZ_CP040309:1038550-1038843 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT125358 NZ_CP040309:1038550-1038843 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6