Detailed information of TA system TA125323

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1038557..1039251
• Replicon: chromosome
• Accession: NZ_CP040307
• Organism: Escherichia coli strain F1 E4

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: FEL01_RS04745
• Protein ID: WP_001263495.1
• Coordinates: 1038853..1039251 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: FEL01_RS04740
• Protein ID: WP_000554757.1
• Coordinates: 1038557..1038850 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEL01_RS04720	1034268..1034765	+	498	Protein_906	REP-associated tyrosine transposase RayT	-
FEL01_RS04725	1034910..1036649	-	1740	WP_001301901.1	flagellar biosynthesis protein FlhA	-
FEL01_RS04730	1036594..1037379	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
FEL01_RS04735	1037450..1038505	+	1056	WP_001226186.1	DNA polymerase IV	-
FEL01_RS04740	1038557..1038850	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FEL01_RS04745	1038853..1039251	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FEL01_RS04750	1039261..1039713	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
FEL01_RS04755	1040032..1040235	+	204	Protein_913	RNA ligase RtcB family protein	-
FEL01_RS04760	1040234..1040755	+	522	Protein_914	peptide chain release factor H	-
FEL01_RS04765	1040812..1042269	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
FEL01_RS04770	1042530..1042988	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	1043584..1043664	+	81	NuclAT_12	-	-
-	1043584..1043664	+	81	NuclAT_12	-	-
-	1043584..1043664	+	81	NuclAT_12	-	-
-	1043584..1043664	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T125323 WP_001263495.1 NZ_CP040307:1038853-1039251 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T125323 NZ_CP040307:1038853-1039251 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT125323 WP_000554757.1 NZ_CP040307:1038557-1038850 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT125323 NZ_CP040307:1038557-1038850 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6