Detailed information of TA system TA125266

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 45273..45537
• Replicon: plasmid pEc1500_CTX
• Accession: NZ_CP040270
• Organism: Escherichia coli strain 1500

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: EST53_RS23200
• Protein ID: WP_001387489.1
• Coordinates: 45385..45537 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	45273..45333 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EST53_RS23185	41375..42445	-	1071	WP_012783932.1	IncI1-type conjugal transfer protein TrbB	-
EST53_RS23190	42464..43672	-	1209	WP_001703845.1	IncI1-type conjugal transfer protein TrbA	-
-	43852..43909	-	58	NuclAT_1	-	-
-	43852..43909	-	58	NuclAT_1	-	-
-	43852..43909	-	58	NuclAT_1	-	-
-	43852..43909	-	58	NuclAT_1	-	-
EST53_RS23195	43979..45070	-	1092	WP_000426061.1	hypothetical protein	-
-	45273..45333	-	61	NuclAT_0	-	Antitoxin
-	45273..45333	-	61	NuclAT_0	-	Antitoxin
-	45273..45333	-	61	NuclAT_0	-	Antitoxin
-	45273..45333	-	61	NuclAT_0	-	Antitoxin
EST53_RS23200	45385..45537	+	153	WP_001387489.1	Hok/Gef family protein	Toxin
EST53_RS23205	45609..45860	-	252	WP_001291964.1	hypothetical protein	-
EST53_RS23210	46160..46456	+	297	WP_011264046.1	hypothetical protein	-
EST53_RS23655	46521..46697	-	177	WP_001054900.1	hypothetical protein	-
EST53_RS23215	47089..47298	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
EST53_RS23220	47370..48032	-	663	WP_012783935.1	plasmid IncI1-type surface exclusion protein ExcA	-
EST53_RS23225	48103..50271	-	2169	WP_015508354.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-15 / blaTEM-1B	-	1..91123	91123

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T125266 WP_001387489.1 NZ_CP040270:45385-45537 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T125266 NZ_CP040270:45385-45537 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT125266 NZ_CP040270:c45333-45273 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin