Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3607778..3608577 Replicon chromosome
Accession NZ_CP040269
Organism Escherichia coli strain 1500

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag EST53_RS17415 Protein ID WP_000347267.1
Coordinates 3607778..3608242 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag EST53_RS17420 Protein ID WP_001307405.1
Coordinates 3608242..3608577 (-) Length 112 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EST53_RS17385 3602779..3603213 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
EST53_RS17390 3603231..3604109 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
EST53_RS17395 3604099..3604878 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
EST53_RS17400 3604889..3605362 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
EST53_RS17405 3605385..3606665 - 1281 WP_000681933.1 tagatose-bisphosphate aldolase subunit KbaZ -
EST53_RS17410 3606914..3607723 + 810 WP_000072193.1 aga operon transcriptional regulator AgaR -
EST53_RS17415 3607778..3608242 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
EST53_RS17420 3608242..3608577 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
EST53_RS17425 3608726..3610297 - 1572 WP_001273741.1 galactarate dehydratase -
EST53_RS17430 3610672..3612006 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
EST53_RS17435 3612022..3612792 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-150)

Antitoxin

(14-110)


Sequences


Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T125262 WP_000347267.1 NZ_CP040269:c3608242-3607778 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T125262 NZ_CP040269:c3608242-3607778 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT125262 WP_001307405.1 NZ_CP040269:c3608577-3608242 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT125262 NZ_CP040269:c3608577-3608242 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4


Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References