125245

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 108735..109237 Replicon chromosome
Accession NZ_CP040269
Organism Escherichia coli strain 1500

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag EST53_RS00525 Protein ID WP_000767829.1
Coordinates 108983..109237 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EST53_RS00520 Protein ID WP_001259255.1
Coordinates 108735..108986 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EST53_RS00495 103913..104980 - 1068 WP_000080108.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
EST53_RS00500 104980..106050 - 1071 WP_000108955.1 histidinol-phosphate transaminase -
EST53_RS00505 106047..107351 - 1305 WP_001349967.1 histidinol dehydrogenase -
EST53_RS00510 107357..108256 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
EST53_RS00515 108402..108452 - 51 WP_001364200.1 his operon leader peptide -
EST53_RS00520 108735..108986 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EST53_RS00525 108983..109237 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EST53_RS00530 109320..110144 + 825 WP_000754762.1 SDR family oxidoreductase -
EST53_RS00535 110190..111119 + 930 WP_150391319.1 LysR family transcriptional regulator -
EST53_RS00540 111334..111396 + 63 WP_010723108.1 membrane protein YoeI -
EST53_RS00545 111386..112744 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EST53_RS00550 112923..113981 + 1059 WP_023568741.1 transport protein YeeE -
EST53_RS00555 113995..114222 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T125245 WP_000767829.1 NZ_CP040269:108983-109237 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T125245 NZ_CP040269:108983-109237 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT125245 WP_001259255.1 NZ_CP040269:108735-108986 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT125245 NZ_CP040269:108735-108986 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References