Detailed information of TA system TA125242

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4988389..4988991
• Replicon: chromosome
• Accession: NZ_CP040263
• Organism: Escherichia coli strain 631

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: EST51_RS24480
• Protein ID: WP_000897305.1
• Coordinates: 4988680..4988991 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EST51_RS24475
• Protein ID: WP_000356395.1
• Coordinates: 4988389..4988679 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EST51_RS24435	4984013..4984915	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
EST51_RS24440	4984912..4985547	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
EST51_RS24445	4985544..4986473	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
EST51_RS24450	4986655..4986897	-	243	WP_001309881.1	ribbon-helix-helix domain-containing protein	-
EST51_RS24455	4987116..4987334	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
EST51_RS24465	4987753..4988031	-	279	WP_001315112.1	hypothetical protein	-
EST51_RS24470	4988083..4988304	-	222	WP_001550354.1	hypothetical protein	-
EST51_RS24475	4988389..4988679	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
EST51_RS24480	4988680..4988991	-	312	WP_000897305.1	hypothetical protein	Toxin
EST51_RS24485	4989220..4990128	+	909	WP_001550353.1	alpha/beta hydrolase	-
EST51_RS24490	4990296..4992110	-	1815	WP_001550352.1	hypothetical protein	-
EST51_RS24495	4992526..4993467	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
EST51_RS24500	4993512..4993949	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T125242 WP_000897305.1 NZ_CP040263:c4988991-4988680 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T125242 NZ_CP040263:c4988991-4988680 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT125242 WP_000356395.1 NZ_CP040263:c4988679-4988389 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT125242 NZ_CP040263:c4988679-4988389 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7