Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2025192..2025694 Replicon chromosome
Accession NZ_CP040263
Organism Escherichia coli strain 631

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag EST51_RS09940 Protein ID WP_000767829.1
Coordinates 2025440..2025694 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EST51_RS09935 Protein ID WP_001259255.1
Coordinates 2025192..2025443 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EST51_RS09910 2020370..2021437 - 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
EST51_RS09915 2021437..2022507 - 1071 WP_000108937.1 histidinol-phosphate transaminase -
EST51_RS09920 2022504..2023808 - 1305 WP_021523144.1 histidinol dehydrogenase -
EST51_RS09925 2023814..2024713 - 900 WP_021523143.1 ATP phosphoribosyltransferase -
EST51_RS09930 2024859..2024909 - 51 WP_001364200.1 his operon leader peptide -
EST51_RS09935 2025192..2025443 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EST51_RS09940 2025440..2025694 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EST51_RS09945 2025777..2026601 + 825 WP_000754758.1 SDR family oxidoreductase -
EST51_RS09950 2026647..2027576 + 930 WP_023154044.1 LysR family transcriptional regulator -
EST51_RS09955 2027791..2027853 + 63 WP_010723108.1 membrane protein YoeI -
EST51_RS09960 2027843..2029201 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EST51_RS09965 2029380..2030438 + 1059 WP_000492324.1 transport protein YeeE -
EST51_RS09970 2030452..2030679 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T125221 WP_000767829.1 NZ_CP040263:2025440-2025694 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T125221 NZ_CP040263:2025440-2025694 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT125221 WP_001259255.1 NZ_CP040263:2025192-2025443 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT125221 NZ_CP040263:2025192-2025443 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References