125221

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2025192..2025694 Replicon chromosome
Accession NZ_CP040263
Organism Escherichia coli strain 631

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag EST51_RS09940 Protein ID WP_000767829.1
Coordinates 2025440..2025694 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EST51_RS09935 Protein ID WP_001259255.1
Coordinates 2025192..2025443 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EST51_RS09910 2020370..2021437 - 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
EST51_RS09915 2021437..2022507 - 1071 WP_000108937.1 histidinol-phosphate transaminase -
EST51_RS09920 2022504..2023808 - 1305 WP_021523144.1 histidinol dehydrogenase -
EST51_RS09925 2023814..2024713 - 900 WP_021523143.1 ATP phosphoribosyltransferase -
EST51_RS09930 2024859..2024909 - 51 WP_001364200.1 his operon leader peptide -
EST51_RS09935 2025192..2025443 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EST51_RS09940 2025440..2025694 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EST51_RS09945 2025777..2026601 + 825 WP_000754758.1 SDR family oxidoreductase -
EST51_RS09950 2026647..2027576 + 930 WP_023154044.1 LysR family transcriptional regulator -
EST51_RS09955 2027791..2027853 + 63 WP_010723108.1 membrane protein YoeI -
EST51_RS09960 2027843..2029201 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EST51_RS09965 2029380..2030438 + 1059 WP_000492324.1 transport protein YeeE -
EST51_RS09970 2030452..2030679 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T125221 WP_000767829.1 NZ_CP040263:2025440-2025694 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T125221 NZ_CP040263:2025440-2025694 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT125221 WP_001259255.1 NZ_CP040263:2025192-2025443 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT125221 NZ_CP040263:2025192-2025443 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References