Detailed information of TA system TA125220

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1223344..1224071
• Replicon: chromosome
• Accession: NZ_CP040263
• Organism: Escherichia coli strain 631

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: EST51_RS06070
• Protein ID: WP_000547555.1
• Coordinates: 1223344..1223655 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EST51_RS06075
• Protein ID: WP_000126294.1
• Coordinates: 1223652..1224071 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EST51_RS06045	1219279..1220988	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
EST51_RS06050	1220998..1221540	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
EST51_RS06055	1221540..1222307	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
EST51_RS06060	1222304..1222714	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
EST51_RS06065	1222707..1223177	+	471	WP_001551542.1	hydrogenase maturation peptidase HycI	-
EST51_RS06070	1223344..1223655	+	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
EST51_RS06075	1223652..1224071	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
EST51_RS06080	1224150..1225574	-	1425	WP_000110355.1	6-phospho-beta-glucosidase AscB	-
EST51_RS06085	1225583..1227040	-	1458	WP_001107889.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
EST51_RS06090	1227300..1228310	+	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
EST51_RS06095	1228459..1228986	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T125220 WP_000547555.1 NZ_CP040263:1223344-1223655 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T125220 NZ_CP040263:1223344-1223655 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCTAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT125220 WP_000126294.1 NZ_CP040263:1223652-1224071 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT125220 NZ_CP040263:1223652-1224071 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCCTTACTTCGTACGCTGATTGATCAACATAATTTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
TCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCATATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: V0YLE2