Detailed information of TA system TA125216

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 736908..737601
• Replicon: chromosome
• Accession: NZ_CP040263
• Organism: Escherichia coli strain 631

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: -
• Locus tag: EST51_RS03575
• Protein ID: WP_000415583.1
• Coordinates: 736908..737204 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: EST51_RS03580
• Protein ID: WP_000650107.1
• Coordinates: 737206..737601 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EST51_RS03540	731996..732310	-	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-
EST51_RS03545	732341..732922	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
EST51_RS03550	733241..733573	+	333	WP_000917684.1	DUF2645 family protein	-
EST51_RS03555	733619..734968	-	1350	WP_000673402.1	two-component system sensor histidine kinase QseC	-
EST51_RS03560	734965..735624	-	660	WP_106106579.1	two-component system response regulator QseB	-
EST51_RS03565	735776..736168	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
EST51_RS03570	736221..736703	+	483	WP_106106578.1	GyrI-like domain-containing protein	-
EST51_RS03575	736908..737204	+	297	WP_000415583.1	type II toxin-antitoxin system toxin MqsR	Toxin
EST51_RS03580	737206..737601	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
EST51_RS03585	737734..739341	+	1608	WP_106106577.1	ABC transporter substrate-binding protein	-
EST51_RS03590	739479..741737	+	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (2-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11216.99 Da
• Isoelectric Point: 8.9070

>T125216 WP_000415583.1 NZ_CP040263:736908-737204 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGLV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T125216 NZ_CP040263:736908-737204 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCTGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT125216 WP_000650107.1 NZ_CP040263:737206-737601 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT125216 NZ_CP040263:737206-737601 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LU65