Detailed information of TA system TA125212

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 176406..176991
• Replicon: plasmid unnamed1
• Accession: NZ_CP040260
• Organism: Acinetobacter baumannii strain P7774

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7S7BY50
• Locus tag: FED54_RS21110
• Protein ID: WP_000897311.1
• Coordinates: 176406..176726 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FED54_RS21115
• Protein ID: WP_000369784.1
• Coordinates: 176719..176991 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FED54_RS21080	172213..172542	+	330	WP_000506885.1	DUF4236 domain-containing protein	-
FED54_RS21090	172814..174046	-	1233	WP_001255535.1	ATP-binding protein	-
FED54_RS21100	174304..174741	-	438	Protein_174	IS3 family transposase	-
FED54_RS21105	174795..175727	+	933	WP_000743272.1	IS5-like element IS17 family transposase	-
FED54_RS21110	176406..176726	+	321	WP_000897311.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FED54_RS21115	176719..176991	+	273	WP_000369784.1	helix-turn-helix domain-containing protein	Antitoxin
FED54_RS21120	177024..177824	+	801	WP_000604996.1	DUF2971 domain-containing protein	-
FED54_RS21125	177915..178216	+	302	Protein_179	transposase	-
FED54_RS21130	178332..178544	+	213	WP_001123161.1	hypothetical protein	-
FED54_RS21135	178870..179726	-	857	Protein_181	LysR family transcriptional regulator	-
FED54_RS21140	179723..180124	-	402	Protein_182	hydroxyacid dehydrogenase	-
FED54_RS21145	180189..180893	+	705	WP_001067790.1	IS6-like element IS1008 family transposase	-
FED54_RS21155	180944..181123	-	180	Protein_184	IS6 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / tet(B) / mph(E) / msr(E) / armA / sul1 / blaPER-7 / qacE / cmlA1 / ARR-3 / aph(6)-Id / aph(3'')-Ib / aac(6')-Ian	-	1..202283	202283
	-	inside	IScluster/Tn	aac(6')-Ian	-	174304..185611	11307

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-84)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12214.03 Da
• Isoelectric Point: 7.9500

>T125212 WP_000897311.1 NZ_CP040260:176406-176726 [Acinetobacter baumannii]
MLFIETSIFTKQIKELVTDEEYRQLQQDLLIQPDKGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETSILRQLVKEQFNG

Nucleotide

• Length: 321 bp

>T125212 NZ_CP040260:176406-176726 [Acinetobacter baumannii]
ATGTTATTCATTGAAACCAGTATTTTTACCAAGCAAATCAAAGAGCTTGTTACTGATGAAGAATATCGTCAACTCCAGCA
AGATCTATTGATACAACCTGATAAAGGAGACTTGATCAAAAATGGTGGTGGCATTCGTAAAGTCCGTTGTGCTCAGGGTA
ATAAAGGCAAAAGTGGCGGTATTCGTGTGATCTATTACTGGGTTACAGAGGATGATCAGATTTTTTTCCTAGTGGCTTAT
CCAAAGTCTGTGAAAGATAATCTGACAGACAAAGAGACCTCGATCCTGCGTCAATTAGTGAAGGAGCAATTTAATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10145.57 Da
• Isoelectric Point: 7.0146

>AT125212 WP_000369784.1 NZ_CP040260:176719-176991 [Acinetobacter baumannii]
MDNNLFDDLVTSIKEAGAIKRNEIKASRVTELELPDIKEVREKTGLTQAEFAARLHISARTLQNWEQGRRYPTGPAATLI
RILDTNPTLI

Nucleotide

• Length: 273 bp

>AT125212 NZ_CP040260:176719-176991 [Acinetobacter baumannii]
ATGGATAACAACTTATTTGATGACCTCGTTACTTCCATCAAAGAAGCTGGAGCCATCAAACGTAATGAGATAAAGGCAAG
TCGAGTAACAGAACTTGAATTGCCTGATATTAAAGAAGTACGTGAGAAAACAGGCTTAACCCAGGCAGAGTTTGCTGCAC
GCTTACATATTAGCGCGAGAACTCTACAAAATTGGGAACAAGGTCGTCGCTATCCGACTGGCCCAGCAGCGACTTTGATT
CGTATTCTTGATACCAATCCAACTCTGATTTAG

Structures

Toxin

• AlphaFold DB: A0A7S7BY50