Detailed information of TA system TA125211

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 142815..143466
• Replicon: plasmid unnamed1
• Accession: NZ_CP040260
• Organism: Acinetobacter baumannii strain P7774

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A4R0DYU1
• Locus tag: FED54_RS20980
• Protein ID: WP_000269907.1
• Coordinates: 143110..143466 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FED54_RS20975
• Protein ID: WP_001140617.1
• Coordinates: 142815..143117 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FED54_RS20940	137853..138635	-	783	WP_213076083.1	replication initiation protein RepM	-
FED54_RS20950	139890..140261	+	372	WP_005174618.1	hypothetical protein	-
FED54_RS21580	140264..140428	+	165	WP_162216716.1	hypothetical protein	-
FED54_RS20955	140471..140857	-	387	WP_001986792.1	helix-turn-helix domain-containing protein	-
FED54_RS20960	141167..141907	+	741	WP_031380848.1	DUF3800 domain-containing protein	-
FED54_RS20965	141972..142274	-	303	WP_001081086.1	hypothetical protein	-
FED54_RS20970	142291..142692	-	402	WP_000660211.1	hypothetical protein	-
FED54_RS20975	142815..143117	-	303	WP_001140617.1	helix-turn-helix transcriptional regulator	Antitoxin
FED54_RS20980	143110..143466	-	357	WP_000269907.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FED54_RS20990	144407..144682	-	276	WP_137987736.1	bacteriocin	-
FED54_RS20995	144695..145000	-	306	WP_137987737.1	hypothetical protein	-
FED54_RS21670	145559..145657	-	99	Protein_156	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / tet(B) / mph(E) / msr(E) / armA / sul1 / blaPER-7 / qacE / cmlA1 / ARR-3 / aph(6)-Id / aph(3'')-Ib / aac(6')-Ian	-	1..202283	202283

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7172

>T125211 WP_000269907.1 NZ_CP040260:c143466-143110 [Acinetobacter baumannii]
MWTVITTDLFNEWLEQQDESTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKGGKKRFYTEMLAIADQQYELHLSTLGEQSNG

Nucleotide

• Length: 357 bp

>T125211 NZ_CP040260:c143466-143110 [Acinetobacter baumannii]
ATGTGGACAGTCATTACGACAGATCTATTTAATGAATGGCTTGAACAGCAAGATGAATCAACACAAGAGAAGGTTTTAGC
TGCTTTGGTTGTTCTGCAACAACAGGGACCGAGCTTAGGCCGCCCTTTAGTAGATACCGTGTATGACTCTAAATTTACCA
ATATGAAAGAGCTTCGTGTTCAACATCGTGGTAAACCACTCCGAGCTTTTTTTGCATTTGATCCATTACGACAGGCCATT
GTGCTGTGTATTGGTGATAAAGGTGGTAAAAAGCGATTCTATACAGAGATGCTGGCGATTGCAGATCAACAATATGAACT
TCATCTCTCAACATTAGGAGAACAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11262.05 Da
• Isoelectric Point: 5.8961

>AT125211 WP_001140617.1 NZ_CP040260:c143117-142815 [Acinetobacter baumannii]
MAKTLQELLANRSPESQARIQKIADELLLENQLYLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT125211 NZ_CP040260:c143117-142815 [Acinetobacter baumannii]
ATGGCTAAAACTTTGCAAGAATTATTAGCTAACCGCTCTCCAGAGAGCCAAGCCCGTATTCAAAAAATAGCTGATGAATT
GCTATTAGAAAATCAGCTTTACCTTATTCGTGAAGAACTCGAAATCTCTCAAAAAGAACTGGCTGAAACTTTGGGAATAA
AACAGCCATCACTTTCAGCAATAGAAAATCGTGGCAATGATCTTAAGATCTCAACCATGAAAAAGTATGTTGAAGCAATG
GGAGGGAAGCTCCGTATTGATGTAGAGCTTCCAACAGGAAAACATATAGGATTCAATGTTTAA

Structures

Toxin

• AlphaFold DB: A0A4R0DYU1