Detailed information of TA system TA125185

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 27648..28357
• Replicon: chromosome
• Accession: NZ_CP040250
• Organism: Mycobacterium avium subsp. hominissuis strain 101174

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: X7V9P5
• Locus tag: FCV17_RS00455
• Protein ID: WP_003873387.1
• Coordinates: 27648..28052 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: X7V856
• Locus tag: FCV17_RS00460
• Protein ID: WP_019732445.1
• Coordinates: 28049..28357 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FCV17_RS00430	22965..23543	-	579	WP_011726026.1	sigma-70 family RNA polymerase sigma factor	-
FCV17_RS00435	23634..24461	-	828	WP_062886661.1	hypothetical protein	-
FCV17_RS00440	24859..25167	+	309	WP_003874400.1	WhiB family transcriptional regulator	-
FCV17_RS00445	25210..25974	+	765	WP_062886662.1	superoxide dismutase family protein	-
FCV17_RS00450	26131..27330	-	1200	WP_081216150.1	FAD-dependent oxidoreductase	-
FCV17_RS00455	27648..28052	+	405	WP_003873387.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FCV17_RS00460	28049..28357	+	309	WP_019732445.1	helix-turn-helix transcriptional regulator	Antitoxin
FCV17_RS00470	28844..30370	-	1527	WP_019732444.1	adenylate/guanylate cyclase domain-containing protein	-
FCV17_RS00475	30465..32081	-	1617	WP_003873390.1	chaperonin GroEL	-
FCV17_RS00480	32170..32472	-	303	WP_019732443.1	co-chaperone GroES	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (32-91)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15503.82 Da
• Isoelectric Point: 10.3815

>T125185 WP_003873387.1 NZ_CP040250:27648-28052 [Mycobacterium avium subsp. hominissuis]
VRKWEVDLTLIEAWMDALDDEEYDNLIAALEQLEEHGPITRRPFVDTLEGSRHPNMKELRPRPTKAGAHIRVLFAFDTRS
RAIMLIAGDKAGNWSKWYAKHIPIADELFDAHQKRLHKAAAKATNRKPRKGKKR

Nucleotide

• Length: 405 bp

>T125185 NZ_CP040250:27648-28052 [Mycobacterium avium subsp. hominissuis]
GTGCGGAAATGGGAGGTTGACCTGACTCTCATCGAAGCCTGGATGGACGCCCTCGATGACGAGGAGTACGACAACTTGAT
AGCAGCGCTCGAGCAGTTGGAGGAACACGGTCCAATTACCCGGCGGCCGTTTGTGGACACCCTTGAAGGGTCACGGCACC
CGAACATGAAGGAGCTCCGTCCGCGCCCCACGAAAGCTGGAGCCCACATTCGCGTGCTATTCGCCTTCGATACCCGGTCG
CGGGCGATCATGTTGATCGCTGGAGACAAGGCGGGGAATTGGTCCAAGTGGTATGCCAAGCACATTCCCATTGCCGACGA
GCTCTTCGATGCTCACCAAAAGCGTTTGCACAAGGCAGCAGCCAAAGCCACAAATCGGAAACCAAGGAAGGGTAAGAAAC
GATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11454.95 Da
• Isoelectric Point: 10.0802

>AT125185 WP_019732445.1 NZ_CP040250:28049-28357 [Mycobacterium avium subsp. hominissuis]
MTNLDDMRRRRPGNRTRIDAIKAEMDREVAHYRLRELREAAGYTQTTLAAAIGVGQNRVSQMEHGDLGTSRVDTLRKYVE
ATGGELEVSVKRPDGSRVLLSL

Nucleotide

• Length: 309 bp

>AT125185 NZ_CP040250:28049-28357 [Mycobacterium avium subsp. hominissuis]
ATGACCAATCTTGATGACATGCGCCGTCGCCGTCCCGGCAACCGGACCCGTATCGACGCGATCAAGGCGGAGATGGACCG
CGAGGTTGCCCACTACCGTCTCCGTGAACTGCGCGAGGCTGCCGGCTACACGCAGACGACTCTCGCTGCGGCTATTGGCG
TCGGGCAGAACCGCGTCTCGCAGATGGAACATGGCGATCTCGGCACCAGCCGGGTCGACACTCTCCGCAAGTACGTGGAA
GCGACTGGGGGTGAGCTGGAAGTGTCCGTCAAGCGTCCGGACGGGTCACGGGTTCTCCTCAGTCTGTAA

Structures

Toxin

• AlphaFold DB: X7V9P5

Antitoxin

• AlphaFold DB: X7V856