Detailed information of TA system TA125172

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 1356163..1356806
• Replicon: chromosome
• Accession: NZ_CP040243
• Organism: Glaesserella parasuis strain HPS-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U4SMB1
• Locus tag: FEF05_RS06710
• Protein ID: WP_005714197.1
• Coordinates: 1356462..1356806 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FEF05_RS06705
• Protein ID: WP_012621770.1
• Coordinates: 1356163..1356459 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEF05_RS06680	1351493..1351741	-	249	WP_016527818.1	hypothetical protein	-
FEF05_RS06685	1351704..1353632	-	1929	WP_149350950.1	ATP-dependent zinc metalloprotease FtsH	-
FEF05_RS06690	1353706..1354332	-	627	WP_010786866.1	23S rRNA (uridine(2552)-2'-O)-methyltransferase RlmE	-
FEF05_RS06695	1354575..1355363	+	789	WP_149350951.1	SDR family oxidoreductase	-
FEF05_RS06700	1355443..1356105	+	663	WP_010786865.1	Fic family protein	-
FEF05_RS06705	1356163..1356459	-	297	WP_012621770.1	helix-turn-helix domain-containing protein	Antitoxin
FEF05_RS06710	1356462..1356806	-	345	WP_005714197.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FEF05_RS06715	1357021..1359000	+	1980	WP_149350952.1	exoribonuclease II	-
FEF05_RS06720	1359111..1360202	+	1092	WP_005714201.1	murein transglycosylase A	-
FEF05_RS06725	1360467..1361094	+	628	Protein_1291	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (52-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-76)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13263.22 Da
• Isoelectric Point: 5.9233

>T125172 WP_005714197.1 NZ_CP040243:c1356806-1356462 [Glaesserella parasuis]
METEEYLTFIETKVFEEDRKALMSDDEYQKFQAYLLESHELGDFIQNTGGCQKIRWKLESNNKGKSGGVRVIYYVVTKQG
KLLLMMMYAKSKQDNMSDKQKAMLKAVVSQLSEE

Nucleotide

• Length: 345 bp

>T125172 NZ_CP040243:c1356806-1356462 [Glaesserella parasuis]
ATGGAAACAGAAGAATATCTTACTTTTATTGAAACCAAAGTTTTTGAAGAAGACCGAAAAGCATTAATGTCTGATGATGA
ATATCAGAAATTTCAGGCTTATTTATTAGAATCTCACGAATTAGGCGATTTTATTCAGAATACTGGTGGTTGCCAAAAAA
TTCGCTGGAAACTAGAAAGTAATAACAAGGGCAAAAGTGGTGGTGTAAGGGTGATTTACTATGTTGTAACCAAACAGGGA
AAATTATTATTAATGATGATGTATGCTAAAAGCAAACAAGACAATATGAGTGATAAACAAAAAGCAATGTTAAAAGCGGT
TGTATCCCAATTAAGTGAGGAGTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11224.13 Da
• Isoelectric Point: 10.0887

>AT125172 WP_012621770.1 NZ_CP040243:c1356459-1356163 [Glaesserella parasuis]
MEKELFERLMESAREMVAIEKGEIEVPPHRIHIHKIPNVKAIRTQHQMKQAEFASLLGVSQNLVQSWEIGRRTPNGAARK
LLAIIEQQPNLVPVLQNL

Nucleotide

• Length: 297 bp

>AT125172 NZ_CP040243:c1356459-1356163 [Glaesserella parasuis]
ATGGAAAAAGAACTTTTTGAACGTCTAATGGAAAGTGCGAGAGAAATGGTCGCCATTGAAAAAGGCGAAATAGAAGTACC
ACCGCATCGTATTCATATCCATAAAATCCCGAATGTCAAAGCGATTAGAACCCAGCACCAGATGAAACAAGCGGAATTTG
CTTCCCTTTTAGGCGTAAGCCAAAACCTTGTGCAGTCGTGGGAAATCGGCAGACGAACGCCAAATGGTGCGGCACGGAAA
TTGCTTGCGATTATTGAACAACAGCCTAATCTTGTCCCTGTACTTCAGAATTTATAA

Structures

Toxin

• AlphaFold DB: U4SMB1