Detailed information of TA system TA125171

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1300574..1301135
• Replicon: chromosome
• Accession: NZ_CP040243
• Organism: Glaesserella parasuis strain HPS-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B8F405
• Locus tag: FEF05_RS06480
• Protein ID: WP_012621703.1
• Coordinates: 1300574..1300852 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: FEF05_RS06485
• Protein ID: WP_005712616.1
• Coordinates: 1300863..1301135 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEF05_RS06465	1296366..1297694	-	1329	WP_149351296.1	exodeoxyribonuclease VII large subunit	-
FEF05_RS06470	1297808..1299070	-	1263	WP_075620774.1	serine hydroxymethyltransferase	-
FEF05_RS06475	1299417..1300427	-	1011	WP_005712612.1	ornithine carbamoyltransferase	-
FEF05_RS06480	1300574..1300852	+	279	WP_012621703.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FEF05_RS06485	1300863..1301135	+	273	WP_005712616.1	HigA family addiction module antidote protein	Antitoxin
FEF05_RS06490	1301199..1302419	+	1221	WP_005712618.1	ATP-dependent RNA helicase RhlB	-
FEF05_RS06495	1302429..1303916	+	1488	WP_062923800.1	DUF853 domain-containing protein	-
FEF05_RS06500	1304040..1304240	+	201	WP_021115966.1	hypothetical protein	-
FEF05_RS06505	1304231..1304514	+	284	Protein_1248	transposase	-
FEF05_RS06510	1304674..1305558	-	885	WP_021115963.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-81)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10384.74 Da
• Isoelectric Point: 9.6620

>T125171 WP_012621703.1 NZ_CP040243:1300574-1300852 [Glaesserella parasuis]
MIISFKHKGLKLFFETGSTAGIQANHASKLAFQLATLNQAKSALDMNAPSWNLHQLQDNLAGHWSIKVSGNWRLTFKFQN
GHAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T125171 NZ_CP040243:1300574-1300852 [Glaesserella parasuis]
ATGATTATTTCATTTAAGCATAAAGGATTGAAATTATTTTTTGAAACAGGATCAACCGCAGGTATTCAAGCAAATCACGC
TAGTAAACTTGCTTTTCAATTAGCCACTCTAAACCAAGCAAAAAGTGCTTTAGATATGAATGCACCTAGCTGGAATTTAC
ACCAATTACAAGATAATTTAGCTGGGCATTGGTCTATCAAGGTAAGTGGAAATTGGCGTTTAACTTTTAAATTTCAAAAT
GGTCACGCTGAAGTCGTGGACTATCAAGATTATCATTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10314.06 Da
• Isoelectric Point: 9.8982

>AT125171 WP_005712616.1 NZ_CP040243:1300863-1301135 [Glaesserella parasuis]
MKMYNPAHPGLILREYIEGYNVTEVAKRLGVTRVTLSRILNGKAAISPEMALRLSQLLKTSPELWLGLQSQYDLWQLEQR
PSFNIQPLFA

Nucleotide

• Length: 273 bp

>AT125171 NZ_CP040243:1300863-1301135 [Glaesserella parasuis]
ATGAAAATGTACAATCCAGCTCACCCAGGGCTTATTTTAAGAGAATATATTGAAGGATATAATGTCACAGAAGTGGCTAA
ACGTTTAGGTGTCACTCGTGTCACGCTCTCTCGTATTTTAAATGGCAAAGCCGCAATTTCACCTGAAATGGCTCTTAGAT
TAAGCCAATTATTAAAAACTAGCCCAGAATTATGGCTTGGTTTACAATCCCAATACGATCTTTGGCAGCTTGAACAACGC
CCAAGTTTTAATATTCAGCCGTTATTTGCATAG

Structures

Toxin

• AlphaFold DB: B8F405