Detailed information of TA system TA125119

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 2779637..2780060
• Replicon: chromosome
• Accession: NZ_CP040230
• Organism: Staphylococcus aureus strain GD1108

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5S9I4Y6
• Locus tag: FD491_RS13645
• Protein ID: WP_001065091.1
• Coordinates: 2779637..2779888 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0E0VQM8
• Locus tag: FD491_RS13650
• Protein ID: WP_000028422.1
• Coordinates: 2779878..2780060 (+)
• Length: 61 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FD491_RS13600	2775197..2776117	+	921	WP_138742486.1	recombinase RecT	-
FD491_RS13605	2776198..2776815	+	618	WP_071621397.1	MBL fold metallo-hydrolase	-
FD491_RS13610	2776816..2777286	+	471	WP_000934764.1	single-stranded DNA-binding protein	-
FD491_RS13615	2777316..2778200	+	885	WP_000148316.1	DnaD domain protein	-
FD491_RS13620	2778207..2778425	+	219	WP_000338530.1	hypothetical protein	-
FD491_RS13625	2778434..2778838	+	405	WP_000401960.1	RusA family crossover junction endodeoxyribonuclease	-
FD491_RS13630	2778851..2779219	+	369	WP_000101274.1	hypothetical protein	-
FD491_RS13635	2779223..2779465	+	243	WP_000131366.1	hypothetical protein	-
FD491_RS13640	2779477..2779644	+	168	WP_001624242.1	hypothetical protein	-
FD491_RS13645	2779637..2779888	+	252	WP_001065091.1	DUF1024 family protein	Toxin
FD491_RS13650	2779878..2780060	+	183	WP_000028422.1	hypothetical protein	Antitoxin
FD491_RS13655	2780053..2780595	+	543	WP_000185660.1	dUTP pyrophosphatase	-
FD491_RS13660	2780632..2780838	+	207	WP_000195803.1	DUF1381 domain-containing protein	-
FD491_RS13665	2780835..2781221	+	387	WP_000592207.1	hypothetical protein	-
FD491_RS13670	2781218..2781367	+	150	WP_000595265.1	transcriptional activator RinB	-
FD491_RS13675	2781367..2781567	+	201	WP_000265043.1	DUF1514 family protein	-
FD491_RS13680	2781595..2782011	+	417	WP_000590122.1	hypothetical protein	-
FD491_RS13685	2782243..2782542	+	300	WP_000988336.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	groEL / hlb	2754500..2782542	28042

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1024 (1-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9349.22 Da
• Isoelectric Point: 3.9473

>T125119 WP_001065091.1 NZ_CP040230:2779637-2779888 [Staphylococcus aureus]
MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEILEGMTNAIQHSVKEGVELDEAVGIMAGQVVYKYEEEQE
NEH

Nucleotide

• Length: 252 bp

>T125119 NZ_CP040230:2779637-2779888 [Staphylococcus aureus]
ATGAATAATCGCGAACAAATTGAACAATCAGTTATAAGTGCTAGTGCGTATAACGGTAATGACACAGAGGGATTACTAAA
AGAGATTGAGGACGTTTATAAGAAAGCGCAAGCGTTTGATGAAATACTTGAGGGAATGACAAATGCTATTCAACATTCAG
TTAAAGAAGGTGTTGAACTTGATGAAGCAGTAGGGATTATGGCAGGTCAAGTTGTCTATAAATATGAGGAGGAACAGGAA
AATGAGCATTAG

Antitoxin

Protein

• Length: 61 a.a.
• Molecular weight: 6741.63 Da
• Isoelectric Point: 4.2658

>AT125119 WP_000028422.1 NZ_CP040230:2779878-2780060 [Staphylococcus aureus]
MSISVGDKVFNPETNSTLEIVQLVGDIRDTHYKLSDGSIISLIDFVVKPIHLIKEEQEND

Nucleotide

• Length: 183 bp

>AT125119 NZ_CP040230:2779878-2780060 [Staphylococcus aureus]
ATGAGCATTAGTGTAGGAGACAAGGTTTTTAATCCAGAAACAAATTCAACTTTAGAAATTGTACAACTTGTTGGCGATAT
TAGAGACACGCATTACAAGTTATCTGACGGATCTATTATTAGTCTTATAGACTTTGTTGTTAAACCAATTCATTTAATCA
AGGAGGAGCAGGAAAATGACTAA

Structures

Toxin

• AlphaFold DB: A0A5S9I4Y6

Antitoxin

• AlphaFold DB: A0A0E0VQM8