Detailed information of TA system TA125059

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 930915..931571
• Replicon: chromosome
• Accession: NZ_CP040182
• Organism: Serratia fonticola strain MS5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8I0CSE1
• Locus tag: FD644_RS04245
• Protein ID: WP_071682470.1
• Coordinates: 931248..931571 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A4P8TYG0
• Locus tag: FD644_RS04240
• Protein ID: WP_071682469.1
• Coordinates: 930915..931205 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FD644_RS04215	926325..927053	-	729	WP_071682464.1	RES family NAD+ phosphorylase	-
FD644_RS04220	927034..927795	-	762	WP_071682465.1	hypothetical protein	-
FD644_RS04225	927921..928742	-	822	WP_071682466.1	helix-turn-helix transcriptional regulator	-
FD644_RS04230	928885..929763	+	879	WP_071682467.1	helix-turn-helix domain-containing protein	-
FD644_RS04235	929942..930862	+	921	WP_071682468.1	L,D-transpeptidase family protein	-
FD644_RS04240	930915..931205	-	291	WP_071682469.1	helix-turn-helix domain-containing protein	Antitoxin
FD644_RS04245	931248..931571	-	324	WP_071682470.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FD644_RS04250	931790..932506	-	717	WP_071682471.1	GntR family transcriptional regulator	-
FD644_RS04255	932741..934666	+	1926	WP_071682472.1	PTS 2-O-a-mannosyl-D-glycerate transporter subunit IIABC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (45-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-80)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12376.19 Da
• Isoelectric Point: 9.2975

>T125059 WP_071682470.1 NZ_CP040182:c931571-931248 [Serratia fonticola]
MEYFEFIETPVFSRQRETLLDDDSFRELQTHLLKYHSDGDTISKTGGCKKIRWNRPGMGKQGGVRVIYYVITRSGKLYLL
IAYPKNAKDDLTESEKAILKALTDKLN

Nucleotide

• Length: 324 bp

>T125059 NZ_CP040182:c931571-931248 [Serratia fonticola]
ATGGAATACTTTGAGTTCATCGAAACCCCAGTTTTTAGCAGGCAACGCGAAACCCTATTAGACGATGATAGCTTTCGTGA
ACTACAAACCCACCTGTTAAAATATCACTCAGACGGCGATACTATAAGTAAAACAGGCGGTTGTAAAAAAATCCGATGGA
ACCGTCCAGGCATGGGGAAACAAGGTGGTGTAAGGGTTATCTATTACGTTATCACCCGCAGCGGCAAGCTTTACCTGCTG
ATTGCTTATCCTAAAAACGCCAAAGACGACTTAACCGAATCCGAGAAAGCGATACTTAAAGCGTTAACGGATAAACTGAA
TTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10900.57 Da
• Isoelectric Point: 6.4836

>AT125059 WP_071682469.1 NZ_CP040182:c931205-930915 [Serratia fonticola]
VDKELFNDLVESMEQMVAIEKGEMQPARVHRHHIPNVKSLREASGMKQEEFAQAVGVSPSLVQSWEQNRRIPSGSSLKIL
LMIERHPTIVNDLLTI

Nucleotide

• Length: 291 bp

>AT125059 NZ_CP040182:c931205-930915 [Serratia fonticola]
GTGGACAAAGAACTTTTTAACGATCTGGTCGAAAGCATGGAACAGATGGTCGCGATTGAAAAAGGCGAAATGCAGCCTGC
TCGTGTCCATCGCCATCATATCCCCAACGTGAAATCACTACGCGAAGCCAGCGGCATGAAGCAGGAAGAGTTTGCGCAGG
CGGTTGGAGTTAGTCCTTCCCTAGTGCAAAGTTGGGAACAAAATCGCCGAATACCCTCCGGCTCTTCACTCAAAATCTTG
CTGATGATTGAGCGCCACCCCACAATTGTTAACGATCTACTCACCATCTAA

Structures

Antitoxin

• AlphaFold DB: A0A4P8TYG0