Detailed information of TA system TA125053

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 286888..287450
• Replicon: chromosome
• Accession: NZ_CP040182
• Organism: Serratia fonticola strain MS5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8H9YD16
• Locus tag: FD644_RS01310
• Protein ID: WP_059199241.1
• Coordinates: 286888..287166 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A8H9YCT7
• Locus tag: FD644_RS01315
• Protein ID: WP_071682694.1
• Coordinates: 287166..287450 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FD644_RS01295	282716..283624	+	909	WP_021181351.1	glycine--tRNA ligase subunit alpha	-
FD644_RS01300	283633..285702	+	2070	WP_071682692.1	glycine--tRNA ligase subunit beta	-
FD644_RS01305	285901..286620	+	720	WP_071682693.1	DUF3053 domain-containing protein	-
FD644_RS01310	286888..287166	+	279	WP_059199241.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FD644_RS01315	287166..287450	+	285	WP_071682694.1	HigA family addiction module antidote protein	Antitoxin
FD644_RS01320	288017..289933	+	1917	WP_071682695.1	PTS mannitol transporter subunit IICBA	-
FD644_RS01325	289996..291144	+	1149	WP_071682696.1	mannitol-1-phosphate 5-dehydrogenase	-
FD644_RS01330	291252..291866	+	615	WP_175415600.1	MltR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-66)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10485.88 Da
• Isoelectric Point: 9.8344

>T125053 WP_059199241.1 NZ_CP040182:286888-287166 [Serratia fonticola]
MIKSFKHKGLRQFFEKGSTAGINTQQIGKIRLRLGVIDAAKDISDINRPGYQLHPLSGDRAGQWSITVTGNWRITFEFIN
GDAYIVNYEDYH

Nucleotide

• Length: 279 bp

>T125053 NZ_CP040182:286888-287166 [Serratia fonticola]
ATGATTAAGAGTTTTAAGCACAAGGGATTGCGACAGTTTTTTGAGAAAGGCTCTACGGCTGGCATCAATACTCAACAGAT
CGGTAAAATTCGTCTGAGGCTTGGTGTAATTGATGCAGCAAAGGACATCAGTGATATCAACCGCCCTGGCTATCAACTTC
ACCCATTGTCGGGTGACCGTGCCGGGCAGTGGTCAATAACCGTAACAGGCAACTGGCGCATCACGTTTGAATTTATCAAC
GGTGATGCCTATATCGTCAACTATGAGGATTATCACTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10137.86 Da
• Isoelectric Point: 8.7051

>AT125053 WP_071682694.1 NZ_CP040182:287166-287450 [Serratia fonticola]
MAMHNPPHPGEIIAETLEELGVGIRVFARALMVAPSTAQRLVAGNAAVSPEMAVKLSIVLGSSPEFWLNLQDNYSLWIAK
KAVNTSQLKRLVTA

Nucleotide

• Length: 285 bp

>AT125053 NZ_CP040182:287166-287450 [Serratia fonticola]
ATGGCTATGCATAACCCCCCGCATCCGGGCGAGATCATCGCTGAAACGCTGGAAGAACTGGGTGTTGGTATCCGCGTATT
TGCCAGGGCGTTGATGGTGGCACCCTCCACCGCACAGCGCTTGGTCGCAGGGAATGCGGCTGTTAGCCCTGAGATGGCGG
TGAAGTTGTCCATAGTGCTGGGCAGCTCCCCTGAATTCTGGCTAAACCTGCAGGATAATTACAGCCTGTGGATCGCCAAA
AAAGCAGTCAATACTTCGCAGCTAAAGCGTTTGGTTACCGCTTGA