Detailed information of TA system TA125035

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 2394448..2395046
• Replicon: chromosome
• Accession: NZ_CP040172
• Organism: Vibrio cholerae strain VC1374

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q8KQX9
• Locus tag: FDZ90_RS11685
• Protein ID: WP_000118545.1
• Coordinates: 2394448..2394771 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FDZ90_RS11690
• Protein ID: WP_000058216.1
• Coordinates: 2394768..2395046 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FDZ90_RS11655	2390161..2390547	-	387	WP_156843795.1	hypothetical protein	-
FDZ90_RS11660	2390544..2391116	-	573	WP_139057167.1	type IV conjugative transfer system lipoprotein TraV	-
FDZ90_RS11665	2391191..2392480	-	1290	WP_001883765.1	TraB/VirB10 family protein	-
FDZ90_RS11670	2392483..2393379	-	897	WP_032467354.1	type-F conjugative transfer system secretin TraK	-
FDZ90_RS11675	2393363..2393989	-	627	WP_000667169.1	hypothetical protein	-
FDZ90_RS11680	2393986..2394267	-	282	WP_000433889.1	type IV conjugative transfer system protein TraL	-
FDZ90_RS11685	2394448..2394771	+	324	WP_000118545.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FDZ90_RS11690	2394768..2395046	+	279	WP_000058216.1	helix-turn-helix domain-containing protein	Antitoxin
FDZ90_RS11695	2395072..2395707	-	636	WP_000033753.1	DUF4400 domain-containing protein	-
FDZ90_RS11700	2395694..2396254	-	561	WP_000546510.1	hypothetical protein	-
FDZ90_RS11705	2396264..2398084	-	1821	WP_000661887.1	conjugative transfer system coupling protein TraD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	sul2 / aph(3'')-Ib / aph(6)-Id / floR / dfrA18	-	2302852..2450894	148042

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12267.26 Da
• Isoelectric Point: 9.4705

>T125035 WP_000118545.1 NZ_CP040172:2394448-2394771 [Vibrio cholerae]
MSWKIDFYDGVEDQILDMPPKIQARMIKLLELMEKHGANLGPPHTESMGDGLFEIRAKAQEGIGRGLFCYLKGNHIYVLH
AFVKKSQKTPKNELNLARDRQKEVQKS

Nucleotide

• Length: 324 bp

>T125035 NZ_CP040172:2394448-2394771 [Vibrio cholerae]
ATGAGCTGGAAGATCGACTTTTACGATGGCGTTGAGGATCAGATCCTCGATATGCCACCCAAAATACAAGCTCGCATGAT
TAAGCTTTTAGAGCTGATGGAAAAGCATGGTGCAAACTTGGGGCCTCCTCATACTGAGTCTATGGGCGATGGGTTATTTG
AAATCCGCGCCAAAGCTCAAGAAGGTATTGGTAGAGGCTTATTTTGCTACTTAAAAGGGAATCACATTTATGTGTTACAC
GCTTTTGTAAAAAAATCTCAAAAGACGCCCAAAAATGAGCTCAATCTTGCCAGAGATAGACAAAAAGAGGTTCAAAAATC
ATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10517.07 Da
• Isoelectric Point: 9.9393

>AT125035 WP_000058216.1 NZ_CP040172:2394768-2395046 [Vibrio cholerae]
MSLQKLKQRALANAEVKNEYDKLESEFSFIDQLLTMRTKAGLTQEQVAERMHTQKSNVSRLEKGNANPSWSTLLKYAHAC
GFELTLKPQKMS

Nucleotide

• Length: 279 bp

>AT125035 NZ_CP040172:2394768-2395046 [Vibrio cholerae]
ATGAGTCTGCAAAAACTGAAACAACGTGCTTTAGCCAATGCTGAAGTTAAGAATGAGTACGATAAGCTTGAGAGTGAGTT
CAGCTTTATTGATCAATTGCTTACCATGAGAACAAAGGCAGGACTTACTCAAGAACAAGTTGCTGAAAGAATGCATACCC
AAAAAAGTAATGTCAGTCGTTTAGAAAAAGGTAATGCCAATCCAAGTTGGTCAACTCTTCTAAAATATGCTCACGCATGT
GGCTTTGAACTAACACTCAAGCCACAAAAAATGAGTTAA

Structures

Toxin

• AlphaFold DB: A0A0X1L383