Detailed information of TA system TA125032

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 10369..11026
• Replicon: plasmid pVC1374
• Accession: NZ_CP040171
• Organism: Vibrio cholerae strain VC1374

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: FDZ90_RS00090
• Protein ID: WP_000270043.1
• Coordinates: 10369..10719 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FDZ90_RS00095
• Protein ID: WP_000124640.1
• Coordinates: 10724..11026 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FDZ90_RS00055	6843..7340	-	498	WP_000062185.1	hypothetical protein	-
FDZ90_RS00060	7343..7831	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
FDZ90_RS00065	7928..8263	+	336	WP_000683476.1	hypothetical protein	-
FDZ90_RS00070	8278..8748	-	471	WP_001281821.1	hypothetical protein	-
FDZ90_RS00075	8741..9112	-	372	WP_000516916.1	hypothetical protein	-
FDZ90_RS00080	9123..9317	-	195	WP_000343597.1	hypothetical protein	-
FDZ90_RS00085	9658..10206	-	549	WP_001061574.1	transcriptional regulator	-
FDZ90_RS00090	10369..10719	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FDZ90_RS00095	10724..11026	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
FDZ90_RS00100	11053..11346	-	294	WP_001239997.1	chromosome segregation protein ParM	-
FDZ90_RS00105	11434..11706	-	273	WP_001043047.1	HU family DNA-binding protein	-
FDZ90_RS00110	11764..12291	-	528	WP_001236377.1	thermonuclease family protein	-
FDZ90_RS00115	12522..13379	-	858	WP_001167032.1	hypothetical protein	-
FDZ90_RS00120	13366..13596	-	231	WP_000972663.1	hypothetical protein	-
FDZ90_RS00125	13596..14114	-	519	WP_000210756.1	nitrite reductase	-
FDZ90_RS00130	14111..14557	-	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
FDZ90_RS00135	14557..14916	-	360	WP_000422768.1	hypothetical protein	-
FDZ90_RS00140	14973..15401	-	429	WP_000591074.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1C / sul2	-	1..140291	140291

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T125032 WP_000270043.1 NZ_CP040171:10369-10719 [Vibrio cholerae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T125032 NZ_CP040171:10369-10719 [Vibrio cholerae]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT125032 WP_000124640.1 NZ_CP040171:10724-11026 [Vibrio cholerae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT125032 NZ_CP040171:10724-11026 [Vibrio cholerae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9