Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-RelB
Location 51876..52412 Replicon plasmid unnamed2
Accession NZ_CP040168
Organism Piscirickettsia salmonis strain Psal-104b

Toxin (Protein)


Gene name yoeB Uniprot ID A0A6I5YBD1
Locus tag Psal104b_RS17540 Protein ID WP_016211910.1
Coordinates 51876..52148 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A0B8U9U2
Locus tag Psal104b_RS17545 Protein ID WP_032126795.1
Coordinates 52152..52412 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Psal104b_RS17505 (Psal104b_03468) 47805..48230 - 426 WP_047927048.1 hypothetical protein -
Psal104b_RS17510 (Psal104b_03469) 48472..49212 + 741 WP_230382708.1 IS30 family transposase -
Psal104b_RS17515 (Psal104b_03470) 49329..49793 + 465 WP_036774631.1 pyrimidine dimer DNA glycosylase/endonuclease V -
Psal104b_RS17520 (Psal104b_03471) 49861..49992 - 132 Protein_52 helix-turn-helix domain-containing protein -
Psal104b_RS17525 50165..50269 + 105 Protein_53 transposase zinc-binding domain-containing protein -
Psal104b_RS17530 (Psal104b_03473) 51028..51159 - 132 Protein_54 helix-turn-helix domain-containing protein -
Psal104b_RS17535 (Psal104b_03474) 51332..51550 + 219 WP_155046644.1 hypothetical protein -
Psal104b_RS17540 (Psal104b_03475) 51876..52148 - 273 WP_016211910.1 Txe/YoeB family addiction module toxin Toxin
Psal104b_RS17545 (Psal104b_03476) 52152..52412 - 261 WP_032126795.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
Psal104b_RS17550 (Psal104b_03477) 52685..53275 + 591 WP_027242600.1 site-specific integrase -
Psal104b_RS17555 (Psal104b_03478) 53303..54706 - 1404 WP_048876031.1 IS4 family transposase -
Psal104b_RS17560 55410..56375 - 966 Protein_60 IS982 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..58980 58980
- flank IS/Tn - - 55738..55932 194


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-88)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-74)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10739.22 Da        Isoelectric Point: 7.8926

>T125031 WP_016211910.1 NZ_CP040168:c52148-51876 [Piscirickettsia salmonis]
MSKLNLLTWSDDAWDDYLYWQDTDKKVLKKINKLIKECQRTPTEGIGHPEPLKHDFSGYWSRKIKGEHRLVYSFDATSLM
IAACRYHYDQ

Download         Length: 273 bp

>T125031 NZ_CP040168:c52148-51876 [Piscirickettsia salmonis]
TTGTCAAAACTAAACTTACTTACTTGGAGTGATGATGCTTGGGATGATTACCTTTATTGGCAAGATACAGATAAAAAGGT
ACTTAAAAAAATCAATAAACTTATAAAGGAATGTCAACGCACGCCGACAGAAGGTATTGGTCATCCCGAACCTTTAAAAC
ATGATTTCAGTGGCTATTGGAGCCGAAAAATAAAGGGTGAGCATAGATTAGTTTATTCATTTGATGCGACCAGTTTAATG
ATTGCTGCATGCCGTTACCATTATGATCAATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9555.93 Da        Isoelectric Point: 5.1167

>AT125031 WP_032126795.1 NZ_CP040168:c52412-52152 [Piscirickettsia salmonis]
MLRDNINATAARDNFKSTCDTVCENNAPVLVKRKGAEDVVIISASDFGGMEETIHLLSSRIMATRLMRSMKQLDEGKGVQ
RDIIEE

Download         Length: 261 bp

>AT125031 NZ_CP040168:c52412-52152 [Piscirickettsia salmonis]
ATGTTACGAGACAACATTAATGCTACGGCAGCGCGGGATAATTTTAAGAGTACCTGTGATACGGTGTGTGAAAACAATGC
CCCTGTACTTGTGAAGCGCAAGGGTGCTGAGGATGTCGTTATTATTTCTGCTTCTGACTTTGGTGGTATGGAAGAAACGA
TCCACTTATTAAGTAGTAGAATAATGGCAACACGCCTTATGAGAAGCATGAAACAACTTGATGAAGGTAAAGGTGTTCAA
CGTGATATTATTGAGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6I5YBD1

Antitoxin

Source ID Structure
AlphaFold DB A0A0B8U9U2

References