Detailed information of TA system TA125026

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5511299..5511894
• Replicon: chromosome
• Accession: NZ_CP040127
• Organism: Pseudomonas aeruginosa strain PA298

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: E6P00_RS28710
• Protein ID: WP_003113526.1
• Coordinates: 5511616..5511894 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E6P00_RS28705
• Protein ID: WP_003113527.1
• Coordinates: 5511299..5511604 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E6P00_RS28670	5506440..5507288	+	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
E6P00_RS28680	5507455..5508396	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
E6P00_RS28685	5508513..5509127	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
E6P00_RS28690	5509169..5509753	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
E6P00_RS28695	5509794..5510894	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
E6P00_RS28705	5511299..5511604	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
E6P00_RS28710	5511616..5511894	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E6P00_RS28720	5512223..5514451	+	2229	WP_003099265.1	TonB-dependent receptor	-
E6P00_RS28725	5514521..5515168	-	648	WP_003095021.1	carbonate dehydratase	-
E6P00_RS28730	5515230..5516468	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T125026 WP_003113526.1 NZ_CP040127:c5511894-5511616 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T125026 NZ_CP040127:c5511894-5511616 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT125026 WP_003113527.1 NZ_CP040127:c5511604-5511299 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT125026 NZ_CP040127:c5511604-5511299 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3