Detailed information of TA system TA125024

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4782878..4783559
• Replicon: chromosome
• Accession: NZ_CP040127
• Organism: Pseudomonas aeruginosa strain PA298

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: E6P00_RS25190
• Protein ID: WP_015503432.1
• Coordinates: 4783194..4783559 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E6P00_RS25185
• Protein ID: WP_016561576.1
• Coordinates: 4782878..4783201 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E6P00_RS25145	4778499..4779377	+	879	WP_003109435.1	hypothetical protein	-
E6P00_RS25155	4779889..4780755	-	867	WP_023081971.1	hypothetical protein	-
E6P00_RS25160	4780785..4781036	-	252	WP_124136459.1	hypothetical protein	-
E6P00_RS25165	4781253..4781807	-	555	WP_074197746.1	tyrosine-type recombinase/integrase	-
E6P00_RS25170	4781981..4782256	-	276	WP_004353146.1	hypothetical protein	-
E6P00_RS25175	4782256..4782588	-	333	WP_016561578.1	hypothetical protein	-
E6P00_RS25180	4782643..4782762	+	120	Protein_4382	IS5/IS1182 family transposase	-
E6P00_RS25185	4782878..4783201	-	324	WP_016561576.1	XRE family transcriptional regulator	Antitoxin
E6P00_RS25190	4783194..4783559	-	366	WP_015503432.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E6P00_RS25195	4783823..4784065	-	243	WP_043884955.1	hypothetical protein	-
E6P00_RS25200	4784272..4784544	+	273	WP_003085667.1	hypothetical protein	-
E6P00_RS25205	4784563..4784988	-	426	WP_003163196.1	VOC family protein	-
E6P00_RS25210	4785089..4785973	+	885	WP_019725847.1	LysR family transcriptional regulator	-
E6P00_RS25215	4785946..4786899	-	954	WP_003085661.1	LysR family transcriptional regulator	-
E6P00_RS25220	4787120..4787554	+	435	WP_003158601.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13798.14 Da
• Isoelectric Point: 4.8219

>T125024 WP_015503432.1 NZ_CP040127:c4783559-4783194 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRPGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDDRWYELNVPIADRLHDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T125024 NZ_CP040127:c4783559-4783194 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCCTG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCCGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCCATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11903.72 Da
• Isoelectric Point: 5.8900

>AT125024 WP_016561576.1 NZ_CP040127:c4783201-4782878 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQSRGLSQKMLSEVLQVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT125024 NZ_CP040127:c4783201-4782878 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGTCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCAGGTGC
AGCAGCCGGCAATTGCCAAGATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCAGCTGGAAGTGATTGCACGCTTCCCGGATGGTGCGGTGAAAATCAGCAACTTCGCGGATATCGAAGAGGCGGGCGC
CTAA