Detailed information of TA system TA125020

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 1262565..1263187
• Replicon: chromosome
• Accession: NZ_CP040127
• Organism: Pseudomonas aeruginosa strain PA298

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: E6P00_RS08470
• Protein ID: WP_023910260.1
• Coordinates: 1262565..1262879 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E6P00_RS08475
• Protein ID: WP_023910258.1
• Coordinates: 1262882..1263187 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E6P00_RS08440	1258227..1259225	+	999	WP_058013018.1	site-specific integrase	-
E6P00_RS08445	1259294..1259605	+	312	WP_031631670.1	outer membrane protein assembly factor BamE	-
E6P00_RS08450	1259737..1260387	+	651	WP_058172679.1	BRCT domain-containing protein	-
E6P00_RS08455	1260485..1260958	+	474	WP_124103543.1	hypothetical protein	-
E6P00_RS08460	1261246..1261989	+	744	WP_058172667.1	DUF3800 domain-containing protein	-
E6P00_RS08470	1262565..1262879	+	315	WP_023910260.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E6P00_RS08475	1262882..1263187	+	306	WP_023910258.1	helix-turn-helix domain-containing protein	Antitoxin
E6P00_RS08480	1263248..1263547	-	300	WP_137461871.1	hypothetical protein	-
E6P00_RS08485	1263648..1263860	-	213	WP_033967225.1	hypothetical protein	-
E6P00_RS08490	1263857..1265737	-	1881	WP_107113824.1	DNA cytosine methyltransferase	-
E6P00_RS08495	1265734..1266213	-	480	WP_137461872.1	glycoside hydrolase	-
E6P00_RS08500	1266223..1266462	-	240	WP_019396882.1	hypothetical protein	-
E6P00_RS08505	1266459..1267085	-	627	WP_058172360.1	hypothetical protein	-
E6P00_RS08510	1267136..1267453	-	318	WP_019396880.1	pyocin activator PrtN family protein	-
E6P00_RS08515	1267450..1267680	-	231	WP_137461873.1	hypothetical protein	-
E6P00_RS08520	1267831..1268124	-	294	WP_023096384.1	phage antirepressor KilAC domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1258269..1299462	41193

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-101)

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-72)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11776.54 Da
• Isoelectric Point: 9.4385

>T125020 WP_023910260.1 NZ_CP040127:1262565-1262879 [Pseudomonas aeruginosa]
MIFIETPVFTKRILALVDDETYRKLQEDLTLHPDAGVVIEGTGGVRKIRIAANGHGKRGGARVIYYHFTSASQIAFLLAY
DKASQEDLTADQKKMLRQIVENWR

Nucleotide

• Length: 315 bp

>T125020 NZ_CP040127:1262565-1262879 [Pseudomonas aeruginosa]
ATGATCTTCATCGAAACGCCAGTCTTCACGAAGCGGATTCTCGCGCTGGTAGACGACGAGACCTATCGCAAGCTTCAGGA
AGACCTAACGCTCCACCCGGACGCCGGAGTGGTCATTGAAGGCACGGGTGGCGTGCGCAAGATTCGCATTGCTGCCAATG
GCCACGGGAAAAGAGGCGGCGCCCGGGTCATCTACTACCATTTCACATCCGCCTCTCAAATTGCATTCCTGCTGGCCTAT
GACAAAGCATCGCAGGAAGATCTGACTGCCGATCAGAAAAAGATGCTTCGGCAGATCGTTGAGAACTGGAGATAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11316.84 Da
• Isoelectric Point: 7.2077

>AT125020 WP_023910258.1 NZ_CP040127:1262882-1263187 [Pseudomonas aeruginosa]
MDKELFAELVESVTQMDEIVRGERQPSREFHVDAVKVKEIRRATGLSQAKFARKIDVAVGTLRNWEQGRRDPEGPARALL
RAIDNDPVHVLAALNGGPRES

Nucleotide

• Length: 306 bp

>AT125020 NZ_CP040127:1262882-1263187 [Pseudomonas aeruginosa]
ATGGACAAAGAACTCTTCGCTGAACTGGTCGAAAGCGTGACCCAGATGGACGAGATCGTGCGTGGCGAACGTCAACCTTC
CCGCGAGTTCCACGTCGACGCGGTGAAGGTCAAGGAAATCCGCCGCGCTACTGGCCTGTCCCAGGCCAAATTCGCCCGAA
AGATCGATGTTGCTGTTGGAACCCTGCGCAATTGGGAGCAAGGGCGCCGCGATCCCGAGGGGCCTGCCCGCGCCCTGCTC
CGGGCCATTGACAATGATCCGGTTCATGTTCTGGCTGCCCTGAATGGTGGCCCGCGAGAAAGCTAA