Detailed information of TA system TA125014

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 32930..33183
• Replicon: plasmid pLSH-KPN148-2
• Accession: NZ_CP040124
• Organism: Klebsiella pneumoniae strain LSH-KPN148

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: FDY04_RS27895
• Protein ID: WP_001312851.1
• Coordinates: 32930..33079 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	33124..33183 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FDY04_RS27860	28289..28704	-	416	Protein_41	IS1 family transposase	-
FDY04_RS27865	28953..29354	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
FDY04_RS27870	29287..29544	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
FDY04_RS27875	29637..30290	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
FDY04_RS27880	31229..32086	-	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
FDY04_RS27885	32079..32153	-	75	WP_001375168.1	RepA leader peptide Tap	-
FDY04_RS27890	32398..32646	-	249	WP_000083837.1	replication regulatory protein RepA	-
FDY04_RS27895	32930..33079	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	33124..33183	+	60	NuclAT_1	-	Antitoxin
-	33124..33183	+	60	NuclAT_1	-	Antitoxin
-	33124..33183	+	60	NuclAT_1	-	Antitoxin
-	33124..33183	+	60	NuclAT_1	-	Antitoxin
FDY04_RS27900	33384..33614	-	231	WP_001736714.1	hypothetical protein	-
FDY04_RS27905	33778..34377	-	600	WP_032083981.1	hypothetical protein	-
FDY04_RS27910	34763..34963	-	201	WP_015059022.1	hypothetical protein	-
FDY04_RS27915	35095..35655	-	561	WP_000139328.1	fertility inhibition protein FinO	-
FDY04_RS27920	35710..36456	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
FDY04_RS27925	36476..36637	-	162	Protein_54	DNA helicase	-
FDY04_RS27930	36701..37405	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / blaTEM-1B / rmtB	-	1..72060	72060
	-	flank	IS/Tn	-	-	36701..37405	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T125014 WP_001312851.1 NZ_CP040124:c33079-32930 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T125014 NZ_CP040124:c33079-32930 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT125014 NZ_CP040124:33124-33183 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin