Detailed information of TA system TA124940

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 373987..374641
• Replicon: plasmid unnamed1
• Accession: NZ_CP040096
• Organism: Pantoea sp. SO10

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FCN45_RS21195
• Protein ID: WP_137387437.1
• Coordinates: 373987..374316 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FCN45_RS21200
• Protein ID: WP_137387163.1
• Coordinates: 374345..374641 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FCN45_RS21175	370155..371090	+	936	WP_137387436.1	ABC transporter permease	-
FCN45_RS21180	371087..371872	+	786	WP_137387160.1	ABC transporter permease	-
FCN45_RS21185	371869..373272	+	1404	WP_137387161.1	ABC transporter ATP-binding protein	-
FCN45_RS21190	373269..373790	-	522	WP_137387162.1	GNAT family N-acetyltransferase	-
FCN45_RS21195	373987..374316	+	330	WP_137387437.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FCN45_RS21200	374345..374641	+	297	WP_137387163.1	helix-turn-helix domain-containing protein	Antitoxin
FCN45_RS21205	374691..375239	-	549	WP_061719009.1	XRE family transcriptional regulator	-
FCN45_RS21210	375342..376016	+	675	WP_137387164.1	AzlC family ABC transporter permease	-
FCN45_RS21215	376006..376326	+	321	WP_137387165.1	AzlD domain-containing protein	-
FCN45_RS21220	376308..376850	-	543	WP_137387166.1	GNAT family N-acetyltransferase	-
FCN45_RS21225	376944..377414	+	471	WP_137387167.1	GNAT family N-acetyltransferase	-
FCN45_RS21230	377458..378426	+	969	WP_101761767.1	magnesium/cobalt transporter CorA	-
FCN45_RS21235	378476..379165	+	690	WP_137387168.1	DJ-1/PfpI family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hcp/tssD	1..744154	744154

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (47-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-82)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12797.80 Da
• Isoelectric Point: 8.4710

>T124940 WP_137387437.1 NZ_CP040096:373987-374316 [Pantoea sp. SO10]
ISYLEFIETRVFSKARKVLLEDDDEFHELQCYLLDHHDLGNTIKDTGGCKKIRWSRSGMGKQGGTRIIYFTRLLSGRIYL
LLIYPKNVKDDLNEDEKAMLKVFTRQINL

Nucleotide

• Length: 330 bp

>T124940 NZ_CP040096:373987-374316 [Pantoea sp. SO10]
ATTAGCTATCTGGAGTTTATCGAAACTCGCGTTTTCTCTAAGGCGCGCAAAGTATTACTTGAAGATGATGACGAATTTCA
CGAATTGCAGTGTTATTTACTTGATCATCACGACTTAGGTAACACGATTAAAGACACCGGCGGTTGTAAGAAAATCCGCT
GGAGTCGCTCAGGCATGGGCAAGCAAGGTGGCACACGAATTATTTATTTCACCAGACTGCTGTCGGGAAGAATTTATTTA
CTGCTCATCTACCCTAAAAACGTTAAAGACGATTTAAACGAGGATGAAAAGGCGATGCTTAAAGTTTTCACAAGGCAAAT
CAATTTGTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11233.14 Da
• Isoelectric Point: 7.2043

>AT124940 WP_137387163.1 NZ_CP040096:374345-374641 [Pantoea sp. SO10]
MDKQLFNDLLESMQEMVAIEKGQEIPETIRVHRHALPDVKLIRKNAGLKQAEFADAMGVSVDLIRSWEQQRRNPTGPALK
MLYLIEQQPLLINALRAI

Nucleotide

• Length: 297 bp

>AT124940 NZ_CP040096:374345-374641 [Pantoea sp. SO10]
ATGGACAAACAACTGTTTAACGATTTACTTGAAAGCATGCAGGAGATGGTCGCGATTGAGAAAGGGCAAGAAATCCCTGA
AACAATCCGCGTCCACCGTCATGCGCTACCCGATGTAAAACTCATCAGAAAAAATGCGGGTCTTAAGCAAGCTGAGTTTG
CTGATGCGATGGGTGTCAGTGTGGATTTGATTCGCAGCTGGGAGCAGCAGCGCCGAAACCCAACGGGCCCAGCCTTAAAA
ATGCTTTATTTAATTGAACAGCAGCCACTTTTGATTAACGCCCTGCGAGCAATCTAA