Detailed information of TA system TA124856

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1196351..1197078
• Replicon: chromosome
• Accession: NZ_CP040067
• Organism: Escherichia coli strain A1_181

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: FDP43_RS05995
• Protein ID: WP_000547564.1
• Coordinates: 1196351..1196662 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FDP43_RS06000
• Protein ID: WP_000126294.1
• Coordinates: 1196659..1197078 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FDP43_RS05965	1191493..1193202	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
FDP43_RS05970	1193212..1193754	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
FDP43_RS05975	1193754..1194521	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
FDP43_RS05980	1194518..1194928	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
FDP43_RS05985	1194921..1195391	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
FDP43_RS05990	1195416..1196177	+	762	WP_001026446.1	hypothetical protein	-
FDP43_RS05995	1196351..1196662	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
FDP43_RS06000	1196659..1197078	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
FDP43_RS06005	1197192..1198616	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
FDP43_RS06010	1198625..1200082	-	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
FDP43_RS06015	1200342..1201352	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
FDP43_RS06020	1201501..1202028	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T124856 WP_000547564.1 NZ_CP040067:1196351-1196662 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T124856 NZ_CP040067:1196351-1196662 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT124856 WP_000126294.1 NZ_CP040067:1196659-1197078 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT124856 NZ_CP040067:1196659-1197078 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5