Detailed information of TA system TA124847

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 1417163..1417818
• Replicon: chromosome
• Accession: NZ_CP040059
• Organism: Coxiella burnetii strain RSA439

Toxin (Protein)

• Gene name: graT
• Uniprot ID: Q83BL3
• Locus tag: FDP44_RS07705
• Protein ID: WP_010958264.1
• Coordinates: 1417480..1417818 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FDP44_RS07700
• Protein ID: WP_010958263.1
• Coordinates: 1417163..1417474 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FDP44_RS07675	1412441..1413118	-	678	WP_010958258.1	cyclin	-
FDP44_RS07685	1413436..1414818	-	1383	WP_010958260.1	cysteine--tRNA ligase	-
FDP44_RS07690	1414809..1416206	-	1398	WP_010958261.1	glutamate--tRNA ligase	-
FDP44_RS07695	1416363..1417094	+	732	WP_010958262.1	UDP-2,3-diacylglucosamine diphosphatase	-
FDP44_RS07700	1417163..1417474	-	312	WP_010958263.1	HigA family addiction module antidote protein	Antitoxin
FDP44_RS07705	1417480..1417818	-	339	WP_010958264.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FDP44_RS07710	1418177..1419322	+	1146	WP_010957722.1	ISAs1 family transposase	-
FDP44_RS07715	1419650..1421323	+	1674	WP_010958265.1	CBU_1493 family Dot/Icm T4SS effector	-
FDP44_RS07720	1421497..1422252	-	756	WP_010958266.1	pyridoxine 5'-phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1418177..1419322	1145

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (23-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (26-72)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 13409.66 Da
• Isoelectric Point: 10.4588

>T124847 WP_010958264.1 NZ_CP040059:c1417818-1417480 [Coxiella burnetii]
MPLTLYIMLDVTRKTVILEVIIKSFKDKYTKYLYKGVSVSKWQAIRKQAERRLQILDSVTSLDDLRSLPSNRFESLRGNR
KGQFSIRINKQWRICFKWINNEPTEVEIVDYH

Nucleotide

• Length: 339 bp

>T124847 NZ_CP040059:c1417818-1417480 [Coxiella burnetii]
TTGCCTCTTACGCTGTATATTATGCTTGATGTAACGCGTAAAACTGTTATCCTTGAAGTGATAATAAAAAGTTTCAAGGA
CAAATACACTAAATATTTATATAAAGGTGTATCCGTTTCAAAATGGCAAGCCATACGAAAGCAGGCGGAAAGACGGCTTC
AGATTTTGGATTCAGTGACATCGTTAGATGATTTGAGAAGTTTGCCAAGCAATCGTTTTGAATCGTTGCGGGGGAATAGA
AAAGGACAATTTAGTATAAGGATTAATAAGCAATGGCGCATTTGTTTTAAGTGGATTAACAATGAGCCAACTGAAGTTGA
AATTGTGGATTATCATTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11458.39 Da
• Isoelectric Point: 10.1699

>AT124847 WP_010958263.1 NZ_CP040059:c1417474-1417163 [Coxiella burnetii]
MKMAANRMRPIHPGEILAEELGFLDKMSANQLAKHLAIPTNRVTAILNGARSITADTALRLAKFFGTTPEFWLNLQDAYD
IKMALKKSGKKIEKEVTPYDQAA

Nucleotide

• Length: 312 bp

>AT124847 NZ_CP040059:c1417474-1417163 [Coxiella burnetii]
ATGAAAATGGCAGCTAATAGAATGCGACCCATACATCCAGGTGAGATCCTTGCTGAAGAGTTGGGGTTTTTAGATAAGAT
GTCAGCTAATCAGTTAGCTAAACATTTAGCAATTCCGACCAACCGAGTGACCGCCATTCTCAATGGCGCGCGATCCATTA
CGGCGGATACCGCGCTACGATTGGCTAAGTTTTTTGGGACGACGCCGGAATTTTGGCTAAATCTCCAAGACGCCTATGAC
ATAAAAATGGCATTGAAAAAGTCCGGCAAAAAAATCGAAAAAGAAGTAACCCCTTACGACCAAGCCGCCTAG

Structures

Toxin

• AlphaFold DB: Q83BL3