Detailed information of TA system TA124839

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 34591..35248
• Replicon: plasmid pIncC-L125
• Accession: NZ_CP040039
• Organism: Klebsiella pneumoniae strain KPC160125

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: FDK27_RS27520
• Protein ID: WP_000270043.1
• Coordinates: 34898..35248 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FDK27_RS27515
• Protein ID: WP_000124640.1
• Coordinates: 34591..34893 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FDK27_RS27465	30216..30644	+	429	WP_000591074.1	hypothetical protein	-
FDK27_RS27470	30701..31060	+	360	WP_000422768.1	hypothetical protein	-
FDK27_RS27475	31060..31506	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
FDK27_RS27480	31503..32021	+	519	WP_000210756.1	nitrite reductase	-
FDK27_RS27485	32021..32251	+	231	WP_000972663.1	hypothetical protein	-
FDK27_RS27490	32238..33095	+	858	WP_001167032.1	hypothetical protein	-
FDK27_RS27500	33326..33853	+	528	WP_001236377.1	thermonuclease family protein	-
FDK27_RS27505	33911..34183	+	273	WP_001043047.1	HU family DNA-binding protein	-
FDK27_RS27510	34271..34564	+	294	WP_001239997.1	chromosome segregation protein ParM	-
FDK27_RS27515	34591..34893	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
FDK27_RS27520	34898..35248	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FDK27_RS27525	35411..35959	+	549	WP_001061574.1	transcriptional regulator	-
FDK27_RS27535	36300..36494	+	195	WP_000343597.1	hypothetical protein	-
FDK27_RS27540	36505..36876	+	372	WP_000516916.1	hypothetical protein	-
FDK27_RS27545	36869..37339	+	471	WP_001281821.1	hypothetical protein	-
FDK27_RS27550	37354..37689	-	336	WP_000683476.1	hypothetical protein	-
FDK27_RS27555	37786..38274	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
FDK27_RS27560	38277..38774	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(42) / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2	-	1..151220	151220

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T124839 WP_000270043.1 NZ_CP040039:c35248-34898 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T124839 NZ_CP040039:c35248-34898 [Klebsiella pneumoniae]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT124839 WP_000124640.1 NZ_CP040039:c34893-34591 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT124839 NZ_CP040039:c34893-34591 [Klebsiella pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9