Detailed information of TA system TA124757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1913183..1913792
• Replicon: chromosome
• Accession: NZ_CP040021
• Organism: Salinivibrio kushneri strain AL184

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: FCN78_RS08975
• Protein ID: WP_077521192.1
• Coordinates: 1913183..1913464 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: FCN78_RS08980
• Protein ID: WP_077659396.1
• Coordinates: 1913478..1913792 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FCN78_RS08945	1908186..1909238	+	1053	WP_106407191.1	histidinol-phosphate transaminase	-
FCN78_RS08950	1909250..1910317	+	1068	WP_077659399.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
FCN78_RS08955	1910317..1910916	+	600	WP_077659398.1	imidazole glycerol phosphate synthase subunit HisH	-
FCN78_RS08960	1910923..1911660	+	738	WP_069361210.1	1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase	-
FCN78_RS08965	1911642..1912415	+	774	WP_069361209.1	imidazole glycerol phosphate synthase subunit HisF	-
FCN78_RS08970	1912412..1913035	+	624	WP_077659397.1	bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE	-
FCN78_RS08975	1913183..1913464	+	282	WP_077521192.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FCN78_RS08980	1913478..1913792	+	315	WP_077659396.1	HigA family addiction module antidote protein	Antitoxin
FCN78_RS08985	1913881..1914876	+	996	WP_077521186.1	UDP-N-acetylglucosamine 4,6-dehydratase (inverting)	-
FCN78_RS08990	1914889..1916034	+	1146	WP_077659476.1	UDP-4-amino-4, 6-dideoxy-N-acetyl-beta-L-altrosamine transaminase	-
FCN78_RS08995	1916038..1916742	+	705	WP_077649303.1	pseudaminic acid cytidylyltransferase	-
FCN78_RS09000	1916739..1917815	+	1077	WP_077659395.1	UDP-2,4-diacetamido-2,4, 6-trideoxy-beta-L-altropyranose hydrolase	-
FCN78_RS09005	1917806..1918384	+	579	WP_077659394.1	UDP-4-amino-4, 6-dideoxy-N-acetyl-beta-L-altrosamine N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (11-89)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10725.18 Da
• Isoelectric Point: 9.0192

>T124757 WP_077521192.1 NZ_CP040021:1913183-1913464 [Salinivibrio kushneri]
MIKSFADKRTQALYSKGTSKRFPTELTVRAARKLEYIDLATQLDDLKVPPGNRLHALSGNRQGQYAIAINKQWRICFRFE
DGDAYDVEVCDYH

Nucleotide

• Length: 282 bp

>T124757 NZ_CP040021:1913183-1913464 [Salinivibrio kushneri]
ATGATCAAATCATTCGCAGATAAACGAACGCAGGCACTATACTCAAAGGGAACGTCAAAGAGGTTCCCTACCGAGCTGAC
GGTACGTGCGGCAAGAAAGTTGGAGTATATCGATCTCGCCACACAGCTCGATGATCTCAAGGTACCGCCCGGTAATCGCC
TTCACGCTTTGTCAGGAAACAGACAAGGGCAATATGCAATAGCGATAAACAAACAGTGGCGCATTTGCTTTCGCTTTGAA
GACGGTGACGCGTATGACGTCGAGGTTTGCGACTACCACTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11605.33 Da
• Isoelectric Point: 5.6864

>AT124757 WP_077659396.1 NZ_CP040021:1913478-1913792 [Salinivibrio kushneri]
MTIFNTTGMQRQPTHPGAMLREDFLPDYGLSVEQLADALGVSRQSMIAVLDERKAVSPELALRLARLFGNSPEFWLNAQR
AVDLWEASQSIKHEVAKIKPLDVA

Nucleotide

• Length: 315 bp

>AT124757 NZ_CP040021:1913478-1913792 [Salinivibrio kushneri]
ATGACGATTTTCAATACAACTGGCATGCAACGCCAACCGACGCATCCTGGAGCCATGTTGCGCGAAGACTTTCTGCCCGA
TTACGGCTTGAGTGTAGAACAATTGGCTGATGCTTTGGGCGTCTCTCGTCAATCGATGATTGCCGTATTGGATGAGCGTA
AAGCCGTGAGCCCGGAGCTGGCGCTTCGTCTCGCTCGTCTGTTTGGTAACTCGCCTGAGTTTTGGCTCAATGCGCAAAGG
GCCGTTGATCTTTGGGAGGCGTCGCAATCAATTAAACACGAGGTAGCAAAAATAAAACCGCTTGATGTCGCTTAA