Detailed information of TA system TA124756

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1086420..1086998
• Replicon: chromosome
• Accession: NZ_CP040021
• Organism: Salinivibrio kushneri strain AL184

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FCN78_RS05215
• Protein ID: WP_077522407.1
• Coordinates: 1086420..1086698 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: FCN78_RS05220
• Protein ID: WP_077659479.1
• Coordinates: 1086708..1086998 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FCN78_RS05190	1081949..1082908	-	960	WP_077658881.1	integron integrase	-
FCN78_RS05195	1083242..1083610	+	369	WP_069362635.1	VOC family protein	-
FCN78_RS05200	1083767..1084525	+	759	WP_077659821.1	restriction endonuclease	-
FCN78_RS05210	1085283..1086305	-	1023	WP_077674137.1	IS110 family transposase	-
FCN78_RS05215	1086420..1086698	+	279	WP_077522407.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FCN78_RS05220	1086708..1086998	+	291	WP_077659479.1	HigA family addiction module antidote protein	Antitoxin
FCN78_RS05225	1087095..1087391	+	297	WP_077659480.1	hypothetical protein	-
FCN78_RS15915	1087514..1087630	+	117	WP_198534765.1	hypothetical protein	-
FCN78_RS05235	1087668..1087883	+	216	WP_198534766.1	BrnA antitoxin family protein	-
FCN78_RS05240	1087990..1088187	-	198	Protein_995	type II toxin-antitoxin system RelE/ParE family toxin	-
FCN78_RS05245	1088427..1089296	+	870	WP_077522413.1	PhzF family phenazine biosynthesis protein	-
FCN78_RS05250	1089431..1089907	+	477	WP_077659481.1	GNAT family N-acetyltransferase	-
FCN78_RS05255	1089999..1090412	+	414	WP_077659482.1	hypothetical protein	-
FCN78_RS05260	1090457..1091143	+	687	WP_077522418.1	GNAT family N-acetyltransferase	-
FCN78_RS05265	1091296..1091634	+	339	WP_077522556.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1083767..1098808	15041
	-	flank	IS/Tn	-	-	1085283..1086305	1022

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (10-64)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10206.67 Da
• Isoelectric Point: 11.0125

>T124756 WP_077522407.1 NZ_CP040021:1086420-1086698 [Salinivibrio kushneri]
MIRSFKHKGLAKFFKSGSTAGIQTAHAKRLRLILGRLNAASGAKDMDLPGLRLHELSGNRSGIWAVTVRGNWRVTFRFED
GDAEVVNDQDCH

Nucleotide

• Length: 279 bp

>T124756 NZ_CP040021:1086420-1086698 [Salinivibrio kushneri]
ATGATTCGAAGCTTTAAACACAAGGGCTTGGCGAAATTCTTCAAGTCCGGCAGCACTGCAGGGATCCAAACCGCTCATGC
AAAGAGGCTTCGGCTTATTCTCGGTAGGCTCAATGCGGCTTCCGGCGCCAAAGATATGGATTTGCCGGGCCTAAGGCTCC
ACGAACTTTCCGGCAACCGGTCGGGAATCTGGGCTGTGACGGTAAGGGGTAACTGGCGCGTCACGTTCCGGTTTGAGGAT
GGAGATGCCGAGGTAGTGAATGACCAAGATTGTCATTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10431.01 Da
• Isoelectric Point: 8.0953

>AT124756 WP_077659479.1 NZ_CP040021:1086708-1086998 [Salinivibrio kushneri]
MLMHNPPHPGEVIRELCLDPLGLSVTAAAEALGVSRKTLSAVLNGKAGVSPEMAIRLSIAFNTSSESWLTQQSQYELSHA
EQHRKDLRVKKLVAAQ

Nucleotide

• Length: 291 bp

>AT124756 NZ_CP040021:1086708-1086998 [Salinivibrio kushneri]
ATGTTGATGCACAACCCTCCCCATCCCGGTGAAGTCATACGAGAGCTTTGCCTAGACCCCCTTGGCCTATCTGTTACAGC
AGCTGCGGAGGCGCTCGGGGTGAGCCGGAAAACGTTAAGCGCCGTTTTGAATGGCAAAGCCGGTGTTAGTCCGGAAATGG
CTATTAGGCTTTCTATTGCTTTTAATACCTCATCGGAGAGCTGGCTAACGCAGCAGTCCCAATATGAACTGTCGCATGCT
GAGCAACACCGTAAAGACCTTAGGGTCAAGAAGCTTGTTGCAGCGCAGTAG