Detailed information of TA system TA124749

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2858242..2858900
• Replicon: chromosome
• Accession: NZ_CP040007
• Organism: Arachnia propionica strain F0700

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: A0A4P8ZSU4
• Locus tag: FBF34_RS12905
• Protein ID: WP_014847604.1
• Coordinates: 2858242..2858601 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: -
• Locus tag: FBF34_RS12910
• Protein ID: WP_041696711.1
• Coordinates: 2858601..2858900 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FBF34_RS12880	2853453..2853752	-	300	WP_014847601.1	HigA family addiction module antidote protein	-
FBF34_RS12885	2853763..2854044	-	282	WP_014847602.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FBF34_RS12890	2854349..2855650	+	1302	WP_014847603.1	hypothetical protein	-
FBF34_RS12895	2855647..2857446	+	1800	WP_051015059.1	tetratricopeptide repeat protein	-
FBF34_RS12905	2858242..2858601	+	360	WP_014847604.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FBF34_RS12910	2858601..2858900	+	300	WP_041696711.1	helix-turn-helix transcriptional regulator	Antitoxin
FBF34_RS12915	2859285..2860040	-	756	WP_014847606.1	transposase	-
FBF34_RS12920	2860613..2860894	+	282	WP_041696713.1	helix-turn-helix domain-containing protein	-
FBF34_RS12925	2861196..2862269	-	1074	WP_014847607.1	restriction endonuclease, SacI family	-
FBF34_RS12930	2862325..2863482	-	1158	WP_041696714.1	DNA (cytosine-5-)-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (11-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-86)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13799.04 Da
• Isoelectric Point: 10.7132

>T124749 WP_014847604.1 NZ_CP040007:2858242-2858601 [Arachnia propionica]
MWEVDVTLIADWLRSLDQTSYEQVIAALEVLRDRGLSLGRPLVDTITASRHRNMKELRPGSIGRSEVRILFAFDPKRMAI
LLIAGDKAGSWTRWYKRNVPLADDLFDKHIRALRKRPEK

Nucleotide

• Length: 360 bp

>T124749 NZ_CP040007:2858242-2858601 [Arachnia propionica]
ATGTGGGAGGTAGACGTCACGTTGATCGCTGACTGGCTCAGGTCACTGGACCAAACCTCCTACGAACAGGTCATCGCTGC
GCTGGAGGTGCTTCGCGATCGTGGTCTTTCACTCGGGCGTCCTCTCGTTGACACCATCACCGCTTCACGGCATCGCAACA
TGAAAGAGCTTCGCCCCGGCTCGATAGGGCGATCCGAGGTTCGCATCCTGTTCGCCTTTGACCCCAAGCGCATGGCGATC
TTGCTGATCGCGGGCGACAAGGCTGGTAGCTGGACTCGGTGGTACAAGAGGAACGTCCCACTGGCCGATGATCTGTTCGA
CAAACATATCCGCGCATTGAGGAAGAGACCAGAAAAGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11031.61 Da
• Isoelectric Point: 7.2036

>AT124749 WP_041696711.1 NZ_CP040007:2858601-2858900 [Arachnia propionica]
MTIDDILAERSVDRSQVEAHKARMLAEVRAYRLRELRENAGLTQAQLAERIGVGQRQISKIEHGDLDTAKIGTIRRYLEA
IGGSLSLEYVAGDLKVVIN

Nucleotide

• Length: 300 bp

>AT124749 NZ_CP040007:2858601-2858900 [Arachnia propionica]
ATGACAATTGACGATATTCTCGCCGAACGCTCCGTGGACCGCAGCCAAGTCGAGGCACACAAAGCCCGGATGCTCGCAGA
AGTTCGCGCCTATCGACTTCGAGAGCTCCGCGAAAATGCTGGATTGACGCAGGCGCAGTTGGCCGAACGCATCGGCGTCG
GGCAGCGTCAAATCTCCAAGATCGAGCACGGCGACCTAGACACCGCGAAGATCGGAACGATCCGCCGCTACCTCGAGGCC
ATCGGCGGCAGCCTCTCACTCGAGTACGTCGCAGGAGACCTCAAAGTCGTGATCAATTGA

Structures

Toxin

• AlphaFold DB: A0A4P8ZSU4