Detailed information of TA system TA124748

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 2853453..2854044
• Replicon: chromosome
• Accession: NZ_CP040007
• Organism: Arachnia propionica strain F0700

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A4V1GCM2
• Locus tag: FBF34_RS12885
• Protein ID: WP_014847602.1
• Coordinates: 2853763..2854044 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FBF34_RS12880
• Protein ID: WP_014847601.1
• Coordinates: 2853453..2853752 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FBF34_RS12855	2849124..2849348	-	225	WP_014847597.1	toxin-antitoxin system toxin component PIN family	-
FBF34_RS12860	2849517..2850746	+	1230	WP_014847598.1	ATP-binding protein	-
FBF34_RS12865	2850868..2851701	-	834	WP_157682480.1	DUF4839 domain-containing protein	-
FBF34_RS15585	2852362..2852514	-	153	WP_014847599.1	hypothetical protein	-
FBF34_RS12875	2852899..2853408	-	510	WP_014847600.1	GNAT family N-acetyltransferase	-
FBF34_RS12880	2853453..2853752	-	300	WP_014847601.1	HigA family addiction module antidote protein	Antitoxin
FBF34_RS12885	2853763..2854044	-	282	WP_014847602.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FBF34_RS12890	2854349..2855650	+	1302	WP_014847603.1	hypothetical protein	-
FBF34_RS12895	2855647..2857446	+	1800	WP_051015059.1	tetratricopeptide repeat protein	-
FBF34_RS12905	2858242..2858601	+	360	WP_014847604.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FBF34_RS12910	2858601..2858900	+	300	WP_041696711.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (5-80)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10976.73 Da
• Isoelectric Point: 10.0040

>T124748 WP_014847602.1 NZ_CP040007:c2854044-2853763 [Arachnia propionica]
MIVSFADRDAERLFQRQRVKRLDPRLHRKALIKLWLLDAVHELDELRVPPGNRLEALKGARIGQHSIRINDQWRICFVWT
PAGPSEVEIVDYH

Nucleotide

• Length: 282 bp

>T124748 NZ_CP040007:c2854044-2853763 [Arachnia propionica]
ATGATCGTCTCCTTCGCCGATCGCGACGCTGAGCGACTGTTCCAAAGGCAGCGCGTCAAGCGCCTTGATCCTCGTCTCCA
CAGGAAGGCGCTCATCAAGTTGTGGTTGTTGGATGCCGTCCACGAACTCGACGAGCTTCGAGTGCCGCCGGGTAACCGGC
TGGAGGCGCTCAAGGGAGCCCGAATCGGTCAGCACAGCATCAGGATCAATGACCAGTGGCGGATATGTTTCGTGTGGACC
CCGGCGGGCCCGAGCGAAGTTGAGATCGTGGACTACCATTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11197.96 Da
• Isoelectric Point: 7.4040

>AT124748 WP_014847601.1 NZ_CP040007:c2853752-2853453 [Arachnia propionica]
MDNKMAPVHPGEILVEEFLNPMGVTQHRLAISIGVPPRRINEIVHGKRRITADTALRLGRFFGMNPQFWVNLQSHYDLEI
GKDALGDELDKIQPLRSVA

Nucleotide

• Length: 300 bp

>AT124748 NZ_CP040007:c2853752-2853453 [Arachnia propionica]
ATGGACAACAAAATGGCTCCGGTTCATCCAGGAGAAATTCTCGTGGAGGAGTTCCTGAATCCGATGGGCGTTACTCAGCA
TCGGCTCGCGATCTCCATTGGGGTGCCGCCCCGCCGAATCAACGAGATTGTGCACGGCAAGCGTCGGATCACGGCTGACA
CAGCATTGCGGCTGGGACGCTTCTTCGGAATGAATCCGCAATTCTGGGTCAACTTGCAATCGCACTACGATCTCGAAATC
GGGAAGGATGCACTGGGCGATGAGCTGGACAAGATTCAGCCTCTGAGATCTGTCGCATGA

Structures

Toxin

• AlphaFold DB: A0A4V1GCM2