Detailed information of TA system TA124737

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 24697..25355
• Replicon: plasmid pTG22182_1
• Accession: NZ_CP039994
• Organism: Acinetobacter baumannii strain TG22182

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: EA674_RS19435
• Protein ID: WP_000312250.1
• Coordinates: 24996..25355 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EA674_RS19430
• Protein ID: WP_001096429.1
• Coordinates: 24697..24996 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EA674_RS19390	20755..21012	+	258	WP_000834292.1	hypothetical protein	-
EA674_RS19395	21017..21589	+	573	WP_000429351.1	hypothetical protein	-
EA674_RS19400	21565..21744	+	180	WP_002081921.1	hypothetical protein	-
EA674_RS19405	21761..22390	+	630	WP_002081923.1	hypothetical protein	-
EA674_RS19410	22430..22939	+	510	WP_001043199.1	hypothetical protein	-
EA674_RS19415	23020..23574	+	555	WP_000790086.1	hypothetical protein	-
EA674_RS19420	23624..24160	+	537	WP_000731977.1	hypothetical protein	-
EA674_RS19430	24697..24996	-	300	WP_001096429.1	helix-turn-helix transcriptional regulator	Antitoxin
EA674_RS19435	24996..25355	-	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EA674_RS19440	25556..26122	+	567	WP_031948745.1	hypothetical protein	-
EA674_RS19445	26171..26353	+	183	WP_000373384.1	hypothetical protein	-
EA674_RS19450	26420..27118	+	699	WP_000873188.1	hypothetical protein	-
EA674_RS19455	27257..27640	+	384	WP_000654347.1	hypothetical protein	-
EA674_RS19460	27707..28465	+	759	WP_001053130.1	hypothetical protein	-
EA674_RS19465	28517..28852	+	336	WP_000654055.1	hypothetical protein	-
EA674_RS19475	29719..30033	+	315	WP_000708713.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..71233	71233

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T124737 WP_000312250.1 NZ_CP039994:c25355-24996 [Acinetobacter baumannii]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T124737 NZ_CP039994:c25355-24996 [Acinetobacter baumannii]
ATGGCTTGGGATGTAGAAACAACAGAACTTTTTGATTCTTGGTTGGCAGAACAAGATGAAAATGCTCAAGATAAAATCTT
AGCGTCTTTGCTTGTTTTGTCAGAACTGGGGCCAAATCTAGGCCGGCCTCATGTTGATACTATTAAAGAATCAAAATATC
CGAACATGAAAGAGATACGTGTGCAAGTTAAAGGTCATCCGATCCGTGGTTTCTTTGCATTTGATCCAGAACGTAAAGCC
ATTGTTTTGTGTGCTGGAGATAAGAAAGGGCTAAATGAAAAAGCCTTTTATAAAGAAATGATCAAGATTGCAGATGAACA
ATATGAGCAATATTTACGTGATAATTACGGAGATAAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT124737 WP_001096429.1 NZ_CP039994:c24996-24697 [Acinetobacter baumannii]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT124737 NZ_CP039994:c24996-24697 [Acinetobacter baumannii]
ATGGCTAAATTAAACGATATAATTTCAAAACGCTCACCTGAAAGCCAACAACGCATCAAGAAAATGGCAAATCAAATGCT
ATTGGATATTAACCTATCGAACCTACGGGAAGAGTTAAAAATATCTCAAGCTGAAATTGCAAAAAATCTAGGTGTAAGCC
AAGCTGCTATTTCAAAACGTGAAAAATTAGGTAAAGATCTTAAGATTTCATCATTAAAAGAATATGTTGAAGCTATGGGT
GGCAAAGTAAGGTTAGATGTTGAGTTACCTAACGGAAAGCATGTTGCTATATCTATTTAA