Detailed information of TA system TA124714

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5716906..5717501
• Replicon: chromosome
• Accession: NZ_CP039988
• Organism: Pseudomonas aeruginosa strain T2436

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: FC629_RS26755
• Protein ID: WP_003113526.1
• Coordinates: 5717223..5717501 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FC629_RS26750
• Protein ID: WP_003133769.1
• Coordinates: 5716906..5717211 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FC629_RS26730	5712504..5713562	+	1059	WP_024914545.1	hypothetical protein	-
FC629_RS26735	5713618..5714481	-	864	WP_024914544.1	integrase domain-containing protein	-
FC629_RS26740	5715083..5716225	-	1143	WP_034074152.1	hypothetical protein	-
FC629_RS26750	5716906..5717211	-	306	WP_003133769.1	HigA family addiction module antidote protein	Antitoxin
FC629_RS26755	5717223..5717501	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FC629_RS26760	5717830..5720058	+	2229	WP_023107900.1	TonB-dependent receptor	-
FC629_RS26765	5720129..5720776	-	648	WP_003095021.1	carbonate dehydratase	-
FC629_RS26770	5720838..5722076	-	1239	WP_034029043.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T124714 WP_003113526.1 NZ_CP039988:c5717501-5717223 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T124714 NZ_CP039988:c5717501-5717223 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGCTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT124714 WP_003133769.1 NZ_CP039988:c5717211-5716906 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT124714 NZ_CP039988:c5717211-5716906 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTACTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3