Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 4650911..4651600 Replicon chromosome
Accession NZ_CP039951
Organism Klebsiella pneumoniae strain 18KM2445b

Toxin (Protein)


Gene name higB Uniprot ID A0A331C6E2
Locus tag FC646_RS22420 Protein ID WP_021469727.1
Coordinates 4650911..4651228 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A6B0N7G3
Locus tag FC646_RS22425 Protein ID WP_020804705.1
Coordinates 4651304..4651600 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FC646_RS22390 4646613..4647122 + 510 WP_020802144.1 GNAT family N-acetyltransferase -
FC646_RS22395 4647132..4648058 + 927 WP_032435210.1 amino acid ABC transporter permease -
FC646_RS22400 4648042..4648821 + 780 WP_002906697.1 amino acid ABC transporter ATP-binding protein -
FC646_RS22405 4648860..4649711 + 852 WP_072198177.1 transporter substrate-binding domain-containing protein -
FC646_RS22410 4649789..4650406 + 618 WP_032425015.1 glutathione S-transferase family protein -
FC646_RS22415 4650477..4650704 + 228 WP_002906690.1 tautomerase PptA -
FC646_RS22420 4650911..4651228 + 318 WP_021469727.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FC646_RS22425 4651304..4651600 + 297 WP_020804705.1 helix-turn-helix domain-containing protein Antitoxin
FC646_RS22430 4651679..4652125 + 447 WP_032435212.1 hypothetical protein -
FC646_RS22435 4652166..4653782 - 1617 WP_004175961.1 carbohydrate porin -
FC646_RS22440 4653826..4655208 - 1383 WP_004151222.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(43-102)

Antitoxin

Cro/C1-type helix-turn-helix domain

(39-75)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12221.10 Da        Isoelectric Point: 10.2217

>T124645 WP_021469727.1 NZ_CP039951:4650911-4651228 [Klebsiella pneumoniae]
MLTFIELQGFSKRRQVLLQDDEFRAFQEILINDPEAGDTISGTGGFRKIRWSRPGMGKRGGVRVIYYNVTKKGRIYLALI
YPKNEQDELNQEQKKMLRQVADLIN

Download         Length: 318 bp

>T124645 NZ_CP039951:4650911-4651228 [Klebsiella pneumoniae]
ATGTTGACTTTTATTGAACTACAAGGTTTCAGTAAGCGACGCCAGGTGCTGTTGCAGGATGATGAATTTCGGGCCTTTCA
GGAAATCCTTATAAACGATCCCGAGGCTGGGGACACAATTTCGGGAACCGGTGGTTTTCGTAAAATTCGCTGGAGTCGTC
CCGGGATGGGGAAGCGCGGCGGTGTACGAGTGATTTACTATAATGTAACGAAAAAGGGAAGAATTTACCTGGCGCTCATC
TATCCTAAAAATGAACAGGATGAGTTAAATCAGGAGCAAAAAAAGATGTTGAGACAAGTGGCTGATTTAATAAACTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11057.93 Da        Isoelectric Point: 6.7277

>AT124645 WP_020804705.1 NZ_CP039951:4651304-4651600 [Klebsiella pneumoniae]
MKDELFADLLASAEEMVRIEKGEQMLMEQHVHTFSEIDVKAIREATGLKQQDFAAAVGVSYELVKSWETKRRHPTGAARK
LLLLLQGNPLIINLLKVV

Download         Length: 297 bp

>AT124645 NZ_CP039951:4651304-4651600 [Klebsiella pneumoniae]
ATGAAAGACGAATTGTTTGCCGATCTGCTGGCGAGTGCAGAAGAAATGGTCAGGATCGAGAAAGGTGAACAAATGCTGAT
GGAGCAGCACGTTCATACCTTCAGCGAAATTGACGTGAAAGCGATACGTGAAGCCACAGGTTTAAAACAGCAGGACTTTG
CAGCGGCCGTTGGCGTTAGCTACGAGTTGGTTAAAAGCTGGGAAACGAAACGACGTCACCCAACCGGTGCAGCCAGAAAG
CTGCTTTTATTGCTTCAGGGTAATCCATTGATTATCAACTTGTTGAAGGTTGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A331C6E2

Antitoxin

Source ID Structure
AlphaFold DB A0A6B0N7G3

References