Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1796731..1797367 Replicon chromosome
Accession NZ_CP039895
Organism Rhizobium pusense strain CFBP5875

Toxin (Protein)


Gene name graT Uniprot ID -
Locus tag CFBP5875_RS22305 Protein ID WP_045533913.1
Coordinates 1796731..1797012 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID -
Locus tag CFBP5875_RS22310 Protein ID WP_112766393.1
Coordinates 1797068..1797367 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFBP5875_RS22285 1792766..1793773 + 1008 WP_137069947.1 iron-siderophore ABC transporter substrate-binding protein -
CFBP5875_RS22290 1793770..1794813 + 1044 WP_137069948.1 iron ABC transporter permease -
CFBP5875_RS22295 1794816..1795850 + 1035 WP_137069949.1 iron chelate uptake ABC transporter family permease subunit -
CFBP5875_RS22300 1795847..1796653 + 807 WP_137069950.1 ABC transporter ATP-binding protein -
CFBP5875_RS22305 1796731..1797012 + 282 WP_045533913.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CFBP5875_RS22310 1797068..1797367 + 300 WP_112766393.1 HigA family addiction module antidote protein Antitoxin
CFBP5875_RS22315 1797387..1798259 - 873 WP_137069951.1 helix-turn-helix transcriptional regulator -
CFBP5875_RS22320 1798392..1799606 + 1215 WP_136883440.1 amino acid ABC transporter substrate-binding protein -
CFBP5875_RS22325 1799676..1800548 + 873 WP_112637286.1 branched-chain amino acid ABC transporter permease -
CFBP5875_RS22330 1800545..1801453 + 909 WP_136883441.1 branched-chain amino acid ABC transporter permease -
CFBP5875_RS22335 1801450..1802178 + 729 WP_136883442.1 ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-93)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(4-97)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10610.04 Da        Isoelectric Point: 9.0298

>T124512 WP_045533913.1 NZ_CP039895:1796731-1797012 [Rhizobium pusense]
MIRSFKNKLTASIDDGSVKKGFPSDMLRRAQQLLTVLDAATTVEDLRSPPGNRLEKLSGDREGQYSIRINKQWRICFVWT
QAGPENVEITDYH

Download         Length: 282 bp

>T124512 NZ_CP039895:1796731-1797012 [Rhizobium pusense]
ATGATCCGTTCTTTCAAAAACAAGCTGACTGCTTCGATTGACGACGGATCTGTGAAGAAGGGATTTCCCTCCGATATGCT
ACGCCGCGCCCAGCAACTGCTCACGGTCCTGGATGCGGCGACTACGGTTGAAGACCTTCGGTCACCGCCCGGTAATCGTT
TGGAAAAGCTTTCCGGCGACCGTGAGGGACAATATTCGATCCGGATCAACAAGCAGTGGCGCATCTGCTTCGTCTGGACG
CAAGCAGGGCCGGAAAACGTGGAAATAACCGACTATCACTAG

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11090.89 Da        Isoelectric Point: 8.5148

>AT124512 WP_112766393.1 NZ_CP039895:1797068-1797367 [Rhizobium pusense]
MTISKSPPIHPGEILRELYLEPLGMSAYALARHLNVPRTRIERIAGEKLGITADTALRLAKFFKTTPEYWLNLQNAFDIK
AAATRLETDLANIKELEAA

Download         Length: 300 bp

>AT124512 NZ_CP039895:1797068-1797367 [Rhizobium pusense]
ATGACAATCAGTAAATCGCCTCCGATCCATCCGGGCGAAATCCTGCGGGAACTCTATCTTGAGCCGCTGGGGATGTCGGC
ATACGCGCTCGCAAGGCACCTCAATGTTCCGCGCACCCGCATAGAACGGATTGCGGGAGAGAAACTTGGTATCACCGCGG
ACACCGCCTTGCGTCTGGCAAAGTTTTTCAAGACCACCCCGGAATATTGGCTGAACTTGCAGAACGCTTTTGACATTAAG
GCGGCTGCAACGAGGCTGGAAACGGACCTTGCGAATATCAAGGAATTGGAAGCCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References