Detailed information of TA system TA124503

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 30746..31282
• Replicon: plasmid unnamed1
• Accession: NZ_CP039869
• Organism: Piscirickettsia salmonis strain Psal-113

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A1L6THY5
• Locus tag: Psal113_RS16355
• Protein ID: WP_036772441.1
• Coordinates: 31010..31282 (+)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A0B8U9U2
• Locus tag: Psal113_RS16350
• Protein ID: WP_032126795.1
• Coordinates: 30746..31006 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Psal113_RS16315	26412..27389	-	978	WP_036771347.1	IS5 family transposase	-
Psal113_RS16320	27464..28114	+	651	WP_155046631.1	hypothetical protein	-
Psal113_RS16325	28198..28404	+	207	Protein_38	IS6 family transposase	-
Psal113_RS16330	28455..28622	-	168	Protein_39	IS6 family transposase	-
Psal113_RS16335	28692..29324	+	633	Protein_40	IS6-like element ISPsa2 family transposase	-
Psal113_RS16340	29390..29956	-	567	Protein_41	IS6 family transposase	-
Psal113_RS18725	30124..30273	-	150	WP_155046632.1	hypothetical protein	-
Psal113_RS16345	30242..30406	-	165	Protein_43	IS6 family transposase	-
Psal113_RS16350	30746..31006	+	261	WP_032126795.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
Psal113_RS16355	31010..31282	+	273	WP_036772441.1	Txe/YoeB family addiction module toxin	Toxin
Psal113_RS16360	31358..32086	+	729	WP_017375910.1	IS6 family transposase	-
Psal113_RS16365	32655..33719	-	1065	WP_174872554.1	IS91 family transposase	-
Psal113_RS18730	33899..34078	-	180	WP_157894752.1	hypothetical protein	-
Psal113_RS18735	34211..34411	-	201	WP_196426542.1	transposase zinc-binding domain-containing protein	-
Psal113_RS16370	34491..35318	+	828	WP_048876208.1	IS30 family transposase	-
Psal113_RS16375	35361..36090	-	730	Protein_51	IS6 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..164167	164167
	-	inside	IScluster/Tn	-	-	10357..41704	31347

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (7-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (5-74)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10785.31 Da
• Isoelectric Point: 7.8926

>T124503 WP_036772441.1 NZ_CP039869:31010-31282 [Piscirickettsia salmonis]
MSKLNLLTWSDDAWDDYLYWQDTDKKVLKKINKLIKECQRMPTEGIGHPEPLKHDFSGYWSRKIKGEHRLVYSFDATSLM
ISACRYHYDQ

Nucleotide

• Length: 273 bp

>T124503 NZ_CP039869:31010-31282 [Piscirickettsia salmonis]
TTGTCAAAACTAAACTTACTTACTTGGAGTGATGATGCTTGGGATGATTACCTTTATTGGCAAGATACAGATAAAAAGGT
GCTTAAAAAAATCAATAAACTTATAAAGGAATGTCAACGCATGCCGACAGAAGGTATTGGTCATCCCGAACCTTTAAAAC
ATGATTTCAGTGGCTATTGGAGCCGAAAAATAAAGGGTGAGCATAGATTAGTTTATTCATTTGATGCGACCAGTTTAATG
ATTTCTGCATGCCGTTACCATTATGATCAATGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9555.93 Da
• Isoelectric Point: 5.1167

>AT124503 WP_032126795.1 NZ_CP039869:30746-31006 [Piscirickettsia salmonis]
MLRDNINATAARDNFKSTCDTVCENNAPVLVKRKGAEDVVIISASDFGGMEETIHLLSSRIMATRLMRSMKQLDEGKGVQ
RDIIEE

Nucleotide

• Length: 261 bp

>AT124503 NZ_CP039869:30746-31006 [Piscirickettsia salmonis]
ATGCTACGAGATAACATCAATGCTACGGCAGCACGGGATAATTTTAAGAGTACCTGTGATACAGTGTGTGAAAACAATGC
TCCTGTACTTGTGAAGCGCAAGGGTGCTGAGGATGTCGTTATTATTTCTGCTTCTGACTTTGGTGGTATGGAAGAAACGA
TCCACTTATTAAGTAGTAGAATAATGGCAACACGCCTTATGAGAAGTATGAAACAACTTGATGAAGGTAAAGGTGTTCAA
CGTGATATTATTGAGGAGTAA

Structures

Toxin

• AlphaFold DB: A0A1L6THY5

Antitoxin

• AlphaFold DB: A0A0B8U9U2