Detailed information of TA system TA124493

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3485447..3486117
• Replicon: chromosome
• Accession: NZ_CP039851
• Organism: Mycobacterium tuberculosis variant bovis strain Danish delta-sapM

Toxin (Protein)

• Gene name: higB
• Uniprot ID: O53332
• Locus tag: FCU27_RS17135
• Protein ID: WP_003899954.1
• Coordinates: 3485447..3485791 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: G0THF6
• Locus tag: FCU27_RS17140
• Protein ID: WP_003899955.1
• Coordinates: 3485788..3486117 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FCU27_RS17100	3480520..3481380	+	861	WP_003416628.1	alpha/beta hydrolase	-
FCU27_RS17105	3481355..3481870	+	516	WP_003899950.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
FCU27_RS17115	3482110..3482403	+	294	WP_003416635.1	hypothetical protein	-
FCU27_RS17120	3482691..3483980	+	1290	WP_003416640.1	ATP-binding protein	-
FCU27_RS17125	3484327..3484761	-	435	WP_003899952.1	type II toxin-antitoxin system VapC family toxin	-
FCU27_RS17130	3484764..3485216	-	453	WP_003899953.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FCU27_RS17135	3485447..3485791	+	345	WP_003899954.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FCU27_RS17140	3485788..3486117	+	330	WP_003899955.1	XRE family transcriptional regulator	Antitoxin
FCU27_RS17145	3486655..3487002	+	348	WP_003899956.1	DUF2384 domain-containing protein	-
FCU27_RS17150	3486999..3487619	+	621	WP_003899957.1	RES family NAD+ phosphorylase	-
FCU27_RS17155	3487779..3489044	-	1266	WP_003909837.1	hypothetical protein	-
FCU27_RS17160	3489212..3489421	+	210	WP_003416778.1	hypothetical protein	-
FCU27_RS17170	3489668..3490702	-	1035	WP_003416786.1	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12692.50 Da
• Isoelectric Point: 5.6920

>T124493 WP_003899954.1 NZ_CP039851:3485447-3485791 [Mycobacterium tuberculosis variant bovis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Nucleotide

• Length: 345 bp

>T124493 NZ_CP039851:3485447-3485791 [Mycobacterium tuberculosis variant bovis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11802.46 Da
• Isoelectric Point: 7.4051

>AT124493 WP_003899955.1 NZ_CP039851:3485788-3486117 [Mycobacterium tuberculosis variant bovis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Nucleotide

• Length: 330 bp

>AT124493 NZ_CP039851:3485788-3486117 [Mycobacterium tuberculosis variant bovis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBK2

Antitoxin

• AlphaFold DB: G0THF6