Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2068156..2068658 Replicon chromosome
Accession NZ_CP039837
Organism Escherichia coli O157:H7 strain USDA5905

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag E3175_RS10025 Protein ID WP_000767829.1
Coordinates 2068404..2068658 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag E3175_RS10020 Protein ID WP_001259255.1
Coordinates 2068156..2068407 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3175_RS09995 2063333..2064400 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
E3175_RS10000 2064400..2065470 - 1071 WP_000109000.1 histidinol-phosphate transaminase -
E3175_RS10005 2065467..2066771 - 1305 WP_001359581.1 histidinol dehydrogenase -
E3175_RS10010 2066777..2067676 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
E3175_RS10015 2067822..2067872 - 51 WP_013009236.1 his operon leader peptide -
E3175_RS10020 2068156..2068407 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
E3175_RS10025 2068404..2068658 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
E3175_RS10030 2068741..2069565 + 825 WP_000754737.1 SDR family oxidoreductase -
E3175_RS10035 2069611..2070540 + 930 WP_000803334.1 LysR family transcriptional regulator -
E3175_RS10040 2070755..2070817 + 63 WP_010723108.1 membrane protein YoeI -
E3175_RS10045 2070807..2072165 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
E3175_RS10050 2072344..2073402 + 1059 WP_000492327.1 transport protein YeeE -
E3175_RS10055 2073416..2073643 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T124343 WP_000767829.1 NZ_CP039837:2068404-2068658 [Escherichia coli O157:H7]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T124343 NZ_CP039837:2068404-2068658 [Escherichia coli O157:H7]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT124343 WP_001259255.1 NZ_CP039837:2068156-2068407 [Escherichia coli O157:H7]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT124343 NZ_CP039837:2068156-2068407 [Escherichia coli O157:H7]
ATGCGTACAATTAGCTACAGCGAAGCGCGCCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References